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Amphiphysin/AMPH Antibody (694145)

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Amphiphysin/AMPH was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Amphiphysin/AMPH Monoclonal Antibody (Catalog # MAB6527) at 15 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
694145
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Amphiphysin/AMPH Antibody (694145) Summary

Immunogen
E. coli-derived recombinant human Amphiphysin/AMPH
Asn204-Gly300
Accession # P49418
Specificity
Detects human Amphiphysin/AMPH in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AMPH
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiphysin/AMPH Antibody (694145)

  • AMPH
  • AMPH1
  • amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen)
  • amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
  • amphiphysin I
  • Amphiphysin

Background

AMPH (Amphiphysin; also AMPH1) is a member of the Amphiphysin family, BAR superfamily of proteins.  Although its calculated MW is only 78 kDa, it runs anomalously in SDS-Page at 125-128 kDa.  It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle.  It appears to form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation.  Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization.  Human AMPH is 695 amino acids (aa) in length.  It contains an N-terminal BAR domain (aa 24-240) that binds lipids, a central region that binds AP2 (aa 322-340) and clathrin (aa 347-386), and a C-terminal SH3 domain (aa 622-695) that binds proline-rich ligands.  There is one known 108 kDa splice variant that shows a deletion of aa 425-466.  Over aa 204-300, human AMPH shares 98% aa identity with mouse AMPH. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AMPH
Uniprot