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alpha-Fetoprotein/AFP Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76275AF350
Alexa Fluor 405
NBP1-76275AF405
Alexa Fluor 488
NBP1-76275AF488
Alexa Fluor 532
NBP1-76275AF532
Alexa Fluor 594
NBP1-76275AF594
Alexa Fluor 647
NBP1-76275AF647
Alexa Fluor 700
NBP1-76275AF700
Alexa Fluor 750
NBP1-76275AF750
Biotin
NBP1-76275B
CoraFluor 1
NBP1-76275CL1
DyLight 350
NBP1-76275UV
DyLight 405
NBP1-76275V
DyLight 488
NBP1-76275G
DyLight 594
NBP1-76275DL594
DyLight 650
NBP1-76275C
DyLight 680
NBP1-76275FR
DyLight 755
NBP1-76275IR
FITC
NBP1-76275F
HRP
NBP1-76275H
Janelia Fluor 525
NBP1-76275JF525
Janelia Fluor 549
NBP1-76275JF549
Janelia Fluor 646
NBP1-76275JF646
Janelia Fluor 669
NBP1-76275JF669
PerCP
NBP1-76275PCP
Unconjugated
NBP1-76275
mFluor Violet 450 SE
NBP1-76275MFV450
mFluor Violet 500 SE
NBP1-76275MFV500
mFluor Violet 610 SE
NBP1-76275MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-76275
BSA Free
NBP1-76275
BSA Free
NBP1-76275-0.025mg
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alpha-Fetoprotein/AFP Antibody [DyLight 550] Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP. The immunogen is located within amino acids 340 - 390 of AFP. Amino Acid Squence: CVHEYSRRHPQLAVS
Predicted Species
Porcine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AFP
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep: (83%), Bovine: (83%)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for alpha-Fetoprotein/AFP Antibody [DyLight 550]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AFP