![Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Al](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-OTI2A9-Azide-and-BSA-Free-Western-Blot-NBP2-70103-img0002.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Al")
| Reactivity | Hu, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI2A9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | AFP |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 66.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
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Secondary Antibodies |
Isotype Controls |
Research Areas for alpha-Fetoprotein/AFP Antibody (NBP2-70103)Find related products by research area.
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| Gene Symbol | AFP |
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Alpha 1 Fetoprotein.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-OTI2A9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70103-img0006.jpg "Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Alpha 1 Fetoprotein.")
![Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-OTI2A9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70103-img0007.jpg "Immunohistochemistry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.")
![Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Alpha 1 Fetoprotein antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-OTI2A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70103-img0004.jpg "Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Alpha 1 Fetoprotein antibody, and then analyzed by flow cytometry.")
![Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-OTI2A9-Azide-and-BSA-Free-Western-Blot-NBP2-70103-img0001.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.")
![Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Analysis of Jurkat cells, using anti-Alpha 1 Fetoprotein antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-OTI2A9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70103-img0003.jpg "Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) - Azide and BSA Free [NBP2-70103] - Analysis of Jurkat cells, using anti-Alpha 1 Fetoprotein antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
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