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alpha-Fetoprotein/AFP Antibody (OTI2A9)

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Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.
Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Alpha 1 Fetoprotein monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Alpha 1 Fetoprotein.
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Staining of paraffin-embedded Human liver tissue using anti-Alpha 1 Fetoprotein mouse monoclonal antibody.
Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Alpha 1 ...read more
Western Blot: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Alpha 1 Fetoprotein (Right lane) cDNA for 48 hrs and lysed. ...read more
Flow Cytometry: alpha-Fetoprotein/AFP Antibody (OTI2A9) [NBP2-03084] - Analysis of Jurkat cells, using anti-Alpha 1 Fetoprotein antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70103AF350
Alexa Fluor 405
NBP2-70103AF405
Alexa Fluor 488
NBP2-70103AF488
Alexa Fluor 532
NBP2-70103AF532
Alexa Fluor 594
NBP2-70103AF594
Alexa Fluor 647
NBP2-70103AF647
Alexa Fluor 700
NBP2-70103AF700
Alexa Fluor 750
NBP2-70103AF750
Allophycocyanin
NBP2-70103APC
Biotin
NBP2-70103B
CoraFluor 1
NBP2-70103CL1
DyLight 350
NBP2-70103UV
DyLight 405
NBP2-70103V
DyLight 488
NBP2-70103G
DyLight 550
NBP2-70103R
DyLight 594
NBP2-70103DL594
DyLight 650
NBP2-70103C
DyLight 680
NBP2-70103FR
DyLight 755
NBP2-70103IR
FITC
NBP2-70103F
HRP
NBP2-70103H
Janelia Fluor 525
NBP2-70103JF525
Janelia Fluor 549
NBP2-70103JF549
Janelia Fluor 646
NBP2-70103JF646
Janelia Fluor 669
NBP2-70103JF669
PE
NBP2-70103PE
PerCP
NBP2-70103PCP
mFluor Violet 450 SE
NBP2-70103MFV450
mFluor Violet 500 SE
NBP2-70103MFV500
mFluor Violet 610 SE
NBP2-70103MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70103
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alpha-Fetoprotein/AFP Antibody (OTI2A9) Summary

Immunogen
Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
Theoretical MW
66.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (OTI2A9)

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AFP
Entrez