Alas1 Antibody

Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human) ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.

Showing Publications 1 - 3 of 3.

Publications using NB100-56415	Applications	Species
Ju Y, Mizutani T, Imamichi Y et al. Nuclear receptor 5A (NR5A) family regulates 5-aminolevulinic acid synthase 1 (ALAS1) gene expression in steroidogenic cells. Endocrinology. 2012-11-01 [PMID: 23024262] (WB) Details: WB: Fig 3L (H295R human adrenocortical carcinoma cells), Fig 3M (Y1 mouse adrenocortical cells, Fig S5C (Y1 cells). IHC (P): Fig 3N-P (rat adrenal gland). Note: The specificity of the ALAS1 IMG-5254A pAb was validated with ALAS1 siRNA by WB in human H295R	WB
Seo YM, Park JI, Jeon MJ et al. Gonadotropin regulation of genes differentially expressed in response to PKCzeta inhibitor during ovulation in the rat. Life Sci. 2009-07-17 [PMID: 19467246] (WB, Rat) Details: WB: Rat ovarian granulosa cells (data described, but not shown).	WB	Rat
Zheng J, Shan Y, Lambrecht RW et al. Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin. Mol Cell Biochem. 2008-12-01 [PMID: 18719978] (WB, Human) Details: WB: Huh-7 human hepatoma cells, Figs 5A, 6, 7. The IMG-5254A ALAS1 pAb detected both precursor (71 kDa) and mature (68 kDa) ALAS1 forms.	WB	Human

Array NB100-56415

• Alas1 Antibodies
• Alas1 Lysates
• Alas1 Protein