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AIF-1/Iba1 Antibody (603102) [Unconjugated]

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AIF1 Antibody was detected in immersion fixed paraffin-embedded sections of human Brain Cortex using Mouse Anti-Human AIF1, Monoclonal Antibody (Catalog # MAB7308) at 5ug/mL at 37 ° Celsius for 8 minutes. Before ...read more
AIF-1/Iba1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIF-1/Iba1 Monoclonal Antibody (Catalog # MAB7308) at 0.5 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, mIF
Clone
603102
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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AIF-1/Iba1 Antibody (603102) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human AIF-1/Iba1
Met1-Pro147
Accession # P55008
Specificity
Detects human AIF-1/Iba1 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
AIF1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.5-25 ug/mL
  • Multiplex Immunofluorescence 5 ug/mL
Publications
Read Publications using
MAB7308 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AIF-1/Iba1 Antibody (603102) [Unconjugated]

  • AIF1
  • AIF-1
  • allograft inflammatory factor 1
  • G1
  • IBA1
  • IbaI
  • interferon gamma responsive transcript
  • Ionized calcium-binding adapter molecule 1
  • IRT1
  • IRT-1
  • Protein G1

Background

Allograft-inflammatory factor 1 (AIF-1), also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed in activated macrophages and is important for the proinflammatory response to ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular smooth muscle and endothelial cells in these areas where it promotes vascular remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue during transplant rejection. It also promotes the proliferation of breast cancer cells and in vivo tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the N-terminal 54 aa of isoform 3. Isoform 2 lacks
aa 1-54 as well as aa 121-147 but also has a 66 aa insertion at position 65.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AIF-1/Iba1 Antibody (MAB7308)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IF, IHC, Immunocytochemistry, Immunohistochemistry.


Filter By Application
IF
(1)
IHC
(1)
Immunocytochemistry
(1)
Immunohistochemistry
(1)
All Applications
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Human
(1)
Mouse
(3)
All Species

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Bioinformatics

Gene Symbol AIF1
Uniprot