![Western Blot: AGO1/2 Antibody [NBP3-18654] - Figure 2. Western blot analysis using the antibody directed against AGO1/2 Whole cell extracts of U2OS cells (40 ug) were analysed by Western blot using the antibody against AGO1/2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/AGO1-2-Antibody-Western-Blot-NBP3-18654-img0001.jpg "Western Blot: AGO1/2 Antibody [NBP3-18654] - Figure 2. Western blot analysis using the antibody directed against AGO1/2 Whole cell extracts of U2OS cells (40 ug) were analysed by Western blot using the antibody against AGO1/2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (in kDa) is shown on the left.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against human AGO1/2 (eukaryotic translation initiation factor 2C1 and 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of both EIF2C1 and EIF2C2. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AGO1 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![ELISA: AGO1/2 Antibody [NBP3-18654] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human AGO1/2 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,500.](http://images.novusbio.com/fullsize/AGO1-2 Antibody-ELISA-NBP3-18654-img0002.jpg "ELISA: AGO1/2 Antibody [NBP3-18654] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human AGO1/2 . The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,500.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")