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Adenosine Deaminase/ADA Antibody (4G4-1C6)

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Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in Jurkat.
Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in Jurkat.
Western Blot: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - ADA monoclonal antibody (M01), clone 4G4-1C6. Analysis of ADA expression in HL-60.
ELISA: Adenosine Deaminase/ADA Antibody (4G4-1C6) [H00000100-M01] - Detection limit for recombinant GST tagged ADA is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4G4-1C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Adenosine Deaminase/ADA Antibody (4G4-1C6) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ADA (AAH40226.1, 1 a.a. ~ 363 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL
Specificity
ADA - adenosine deaminase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ADA
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Adenosine Deaminase/ADA Antibody (4G4-1C6)

  • ADA
  • ADA1
  • adenine deaminase
  • Adenosine aminohydrolase
  • Adenosine Deaminase
  • EC 3.5.4.4

Background

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ADA
Entrez
OMIM
Uniprot