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ADAM15 Antibody (007)

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Immunocytochemistry/ Immunofluorescence: ADAM15 Antibody (007) [NBP2-89546] - Staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and ...read more
Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human heart showing blood vessel staining (red arrow) (1:200).
Flow Cytometry: ADAM15 Antibody (007) [NBP2-89546] - Profile of anti-ADAM15 reactivity on MCF-7 cells.
Immunohistochemistry-Paraffin: ADAM15 Antibody (007) [NBP2-89546] - Staining of human lymphonode showing membrane staining of endothelial cells (red arrow) (1:2000).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, ELISA
Clone
007
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ADAM15 Antibody (007) Summary

Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ADAM15 (Accession#: NP_997074.1; Met 1-Thr 696).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ADAM15
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:10000
  • Flow Cytometry 1:25-1:100
  • Immunocytochemistry/ Immunofluorescence 1:20-1:100
  • Immunohistochemistry 1:1000-1:4000
  • Immunohistochemistry-Paraffin 1:1000-1:4000
  • Sandwich ELISA Detection 1:1000-1:10000
Application Notes
This antibody will detect Human ADAM15 in ELISA pair set (Catalog: # NBP2-79482). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89548).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Alternate Names for ADAM15 Antibody (007)

  • ADAM 15
  • ADAM metallopeptidase domain 15
  • ADAM15
  • disintegrin-like, and cysteine-rich protein 15
  • EC 3.4.24
  • MDC15
  • MDC-15
  • Metargidin

Background

ADAM15 is encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ADAM15