Actin (Muscle Specific) Antibody (HHF35)

SDS extract of human myocardium.

Cytoplasmic

Muscle Cell Marker

This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

IgG1 Kappa

Monoclonal

Mouse

ACTA1

Protein A or G purified

Test in a species/application not listed above to receive a full credit towards a future purchase.

Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.).

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.