| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80561 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48154 | |||
| Azide and BSA Free | NBP2-34634-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47662-0.1mg | |||
| IHC-Prediluted | NBP2-48189 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48194 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47663-0.1mg | |||
| IHC-Prediluted | NBP2-48194 | |||
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0019.jpg "Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Total protein from HeLa, 3T3, PC12 and Bovine normal tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Actin in 5% blocking buffer and detected with an anti-mouse IgM secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 5 ug/mL overnight at 4C and detected with an anti-Mouse IgM DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0018.jpg)
![Simple Western: Actin Antibody (mAbGEa) [NB100-74340] - Image shows a specific band for Actin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Simple-Western-NB100-74340-img0014.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0005.jpg)
![Flow Cytometry: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of HeLa cells using mouse Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Flow-Cytometry-NB100-74340-img0015.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Increased relative abundance of Park7 in Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for the Park7 protein. Pooled liver protein extracts (n = 4 livers per pool) were separated by SDS PAGE in multiple replicates (n = 7) and transferred onto nitrocellulose membrane. Membranes were probed with an anti-Park7 antibody using an anti-actin antibody as a loading control. Liver protein extracts were the same as those used for Figure 4(a) as well as fresh liver protein extracts from new mice of the same age, sex, and genotype. Image collected and cropped by CiteAb from the following publication (null), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Actin Antibody (mAbGEa) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0021.jpg)
![Flow (Intracellular): Actin Antibody (mAbGEa) [NB100-74340] - An intracellular stain was performed on A549 cells with NB100-74340 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse IgM Alexa Fluor 488-conjugated secondary antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Flow-Intracellular-NB100-74340-img0020.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin expression in 2) HeLa, 3) NTERA-2, 4) A431, 5) HepG2, 6) MCF7, 7) NIH-3T3, 8) PC-12 and 9) COS-7 whole cell lysates.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0008.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Jurkat and CHO cell lysates using actin antibody [NB100-74340] at 1:100.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Western-Blot-NB100-74340-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - Actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB100-74340) at 1:1800. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (mAbGEa) [NB100-74340] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Actin (mAbGEa) at 2 ug/mL overnight at 4C and detected with an anti-mouse IgM DyLight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunocytochemistry-Immunofluorescence-NB100-74340-img0017.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Actin on human breast cancer tissue using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0010.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (mAbGEa) [NB100-74340] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.](http://images.novusbio.com/fullsize/Actin-Antibody-mAbGEa-Immunohistochemistry-Paraffin-NB100-74340-img0004.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Lp(a) upregulates GPx1 & Prdx6 expression in human HepG2 cells. HepG2 cells were treated with 5 μg/mL of Lp(a) or LDL for 6 hours at 37°C. Western blots of cell lysates were performed with an anti-Gpx1 antibody (a), an anti-Prdx6 antibody (b), an anti-Sod1 antibody (c), & an anti-Park7 antibody (d) using an anti-actin antibody as a loading control. Representative blots are shown. Protein levels were normalized against actin & expressed relative to that of untreated cells. Results are expressed as mean ± SEM for pooled triplicate incubations run in quadruplicate. ∗P<0.05, relative to untreated HepG2 cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30596094), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-310202415392542.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - DenA deneddylase activity.(A) Recombinant human DEN1 & fungal DenA deneddylate a human CUL1-Nedd8 substrate in vitro. SDS-PAGE & subsequent western analysis show cleavage of the substrate (∼60 kDa) producing the C-terminal CUL1 (∼50 kDa) as outlined in experimental procedures. (B) Deneddylation test in a heterologous yeast system. A. nidulans DenA can remove Rub1 from CulD in heterologous expression experiments in S. cerevisiae. DenA expressed as native protein or C-terminally fused w/ a V5/His6 epitope tag. Both variants driven by the inducible GAL1 promoter. CulD, N-terminal-fused w/ the LexA activation domain, expressed under control of the constitutive ADH promoter. A. nidulans proteins expressed in S. cerevisiae wild type & delta csn5 background. Western analysis w/ antibodies against Rub1 ( alpha -Rub1), the LexA epitope ( alpha -LexA) & the V5 epitope ( alpha -V5) performed. Detection w/ alpha -Rub1 generated two additional signals upon culD expression, representing LexA-CulD & a second CulD pool where LexA unspecifically cleaved off. Both signals disappeared upon co-expression of DenA indicating deneddylation activity (red arrows). The slower migrating signal of alpha -LexA western experiments corresponded to Rub1 modified LexA-CulD. This signal absent when DenA co-expressed. Detection of the V5 tag applied to monitor DenA expression. The neddylated yeast cullin migrating at around 100 kDa not affected by DenA activity. (C) Deneddylation of fungal CulA by CSN & DenA. Whole cell lysates of A. nidulans wild type, delta csnE & delta denA probed w/ alpha -CulA. The ratio of neddylated CulA (CulA*N8; ∼106 kDa*) to non-neddylated CulA (∼96 kDa**) calculated from three independent experiments. Membranes reprobed w/ alpha -Actin ( alpha -ACT) for normalization. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003275), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-31020241682162.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Fibrillar adhesions & FN fibrils are dynamic structures governed by Rab21 & PPFIA1.(a) Living siCTL & siRAB21 ECs incubated w/ IST9 mAb for 30 min, fixed, acid washed & stained. RAB21 silencing impairs ED-A FN endocytosis as revealed by decrease of IST9 punctae co-localizing w/ endosome marker EEA1. Right panels are magnifications of boxed areas in left panels. Data are mean value±s.e.m., n=70 cells per condition pooled from two independent experiments. ***P<0.001. (b) Left panel, WB analysis of PPFIA1, RAB21 & actin on total lysates of siCTL, siPPFIA1 & siPPFIA1+siRAB21 ECs. Right panel, WB analysis of insoluble matrix fraction of ECs extracted w/ DOC buffer. PPFIA1 silencing dramatically reduces amount of DOC-insoluble fraction of endogenous ED-A FN. Of note, simultaneous silencing of Rab21 GTPase (siPPFIA1+siRAB21), which drives integrin endocytosis, rescues defective incorporation of endogenous ED-A FN in DOC-insoluble fraction of siPPFIA1 ECs. (c) Confocal microscopy analysis of patterning of endogenous cellular ED-A FN (green) in fixed confluent ECs. Before fixation, living ECs incubated for 20 min w/ exogenous SNAKA51 (red). ED-A FN polymerizes into a fibrillar network in siCTL, but not in siPPFIA1 ECs. Simultaneous silencing of Rab21 GTPase (siPPFIA1+siRAB21) fully restores ED-A FN polymerization in siPPFIA1 ECs. SNAKA51+ active alpha 5 beta 1 integrin localizes in fibrillar adhesion in siCTL, but not in siPPFIA1 ECs. Notably, simultaneous Rab21 (siPPFIA1+siRAB21) silencing promotes localization of SNAKA51 in fibrillar adhesion of siPPFIA1 ECs. Data are mean±s.e.m., n=20 cells per condition pooled from two independent experiments. Scale bar, 20 μm (a,c, right), 50μm (c, left). ***P<0.001; Student's t-test. Image collected & cropped by CiteAb from following publication (//www.nature.com/articles/ncomms13546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-31020241554546.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Effect of the four most common herbal formulas & single herbs on the phosphorylation of myosin light chain (MLC) protein.Briefly, A10 cells were treated with herbal formulas (A) or single herbs (B). Y27632 (Y10; 10 μM) & calyculin A (A50; 50 μg/ml) were used as negative & positive controls. Western blot analysis & staining with anti-phospho-MLC, anti-total-MLC, & anti-beta actin antibodies was then performed. Phospho-MLC, total-MLC, & beta actin were all obtained with their appropriate protein size bands. The relative Phospho-MLC intensity (%) was expressed as [(Phospho-MLC/total-MLC)drug treated/ (Phospho-MLC/total-MLC)cell only x 100%]. The Mean±SEM values for at least three independent experiments along with the representative western blot were performed. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-310202415533828.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - CSN targets DEN1/DenA for degradation in fungal & human cells.(A) Quantitative analysis of repeated western blot experiments displayed the differences in DenA abundance in fungal wild type & delta csnG cells. DenA levels of the different asexual developmental time points are shown relative to the vegetative (veg.) control for each strain. Anti-Actin was applied as loading control (statistics: 2-way ANOVA; n = 3; *p<0,05, **p<0,01). (B) Xpress-DEN1 was overexpressed in siGFP & siCSN1 human cells & steady state Xpress-DEN1 levels were estimated by western analysis with the alpha -Xpress antibody. Xpress-CSN1 was overexpressed in siCSN1 cells & DEN1 & CSN8 were probed with appropriate antibodies. (C) Xpress-DEN1 was overexpressed in siGFP human cells & the proteasome inhibitor MG132 was added 6 h before cell lysis at a final concentration of 10 µM. Cyclohexamide (CHX) was added in a final concentration of 10 µg/ml (D) Xpress-DEN1 was co-expressed in HeLa cells together with Xpress-CSN1wt, Xpress-CSN1(1–221) or Xpress-CSN1(222–527) in the absence or in the presence of MG132 (right panel), which was added 6 h before cell lysis. Cells were lyzed 24 h after co-transfection & lysates were analyzed by western blot using the alpha -DEN1 antibody (0 = only Xpress-DEN1). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1003275), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-31020241682157.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Analysis of Ia production by engineered S. cerevisiae.(A) Analysis of the synthesis of Ia in S. cerevisiae strains, producing actin-R177K, E270D & E270Q. Yeast strains, producing actin-R177K, E270D or E270Q & transformed with the Ia-containing plasmid (Ia) or the vector alone (Vector), were cultivated in SGal for 20 h at 30°C. Cells were broken by glass beads treatment & analyzed by 32P-ADP-ribosylation in the presence of additionally added purified wild type yeast actin (1 μg). Labeled bands represent modified yeast actin & confirm intracellular production of functionally active Ia by the S. cerevisiae strains. (B) Production of Ia by the wild type S. cerevisiae strain. Wild type yeast strains harboring the Ia-containing plasmid (Ia) or the control vector (vector) were cultivated in glucose-containing liquid medium until OD595 = 0.5. Afterwards, glucose was replaced by galactose & cultivation continued for 9 h at 30°C. Cells were lysed & the resulting extract preparations were ADP-ribosylated in the presence of Ia (+ Ia), TccC3 toxin of P. luminescens [42] (+ TccC3), purified muscle actin (+ alpha -actin) or tested in Western blotting with the anti-actin serum to show equal actin concentrations in the samples. (C, D) Mass spectrometry of actin variants. MALDI-TOF MS of wild type (C) & actin-R177K (D) protein variants isolated from S. cerevisiae. Spectra demonstrate disappearance of R177- & appearance of K177-containing peptide in mass analysis (substituted amino acid residue within identified peptides is shown in red). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-31020241612620.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - alpha 5 beta 1 regulates ED-A FN secretion & polymerization.(a) Western blot analysis of lysates of ECs control (siCTL) & alpha 5 integrin subunit (siITGA5) silenced ECs. Cells were lysed 24 hours after the second siRNA oligofection & proteins were separated by SDS–PAGE & probed for alpha 5 integrin subunit or actin (for control purposes). (b) Confocal microscopy analysis of IST9 mAb-labelled endogenous ED-A FN (green) in confluent ECs. ED-A FN polymerizes into a fibrillar network in siCTL, but not in siITGA5 ECs in which it accumulates in the TGN46+ (red) TGN cisternae. The relative amount of fibrillar ED-A FN area was calculated in siCTL & siITGA5 ECs. Data are mean±s.e.m., n=20 cells per condition pooled from two independent experiments. ***P<0.001; Student's t-test. (c) Western blot analysis of soluble ED-A FN released by confluent ECs seeded on Transwell inserts. An equal percentage of apical & basolateral volumes of medium were collected after 72 h of culture, from different wells of siCTL or siITGA5 ECs. Equal amounts of exogenous rabbit IgG were added to samples (spike normalization) for loading control purposes. Quantification of the ratio between apical or basolateral amount of ED-A FN released by siCTL over siITGA5 ECs. alpha 5 integrin subunit silencing impairs basolateral, but not apical ED-A FN secretion. Data are mean±s.e.m., n=8 wells per condition pooled from four independent experiments. **P<0.01; Student's t-test. Scale bar, 50 μm (b). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13546), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-310202415535157.jpg)
![Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - Effect of the four most common herbal formulas & single herbs on the phosphorylation of myosin light chain (MLC) protein.Briefly, A10 cells were treated with herbal formulas (A) or single herbs (B). Y27632 (Y10; 10 μM) & calyculin A (A50; 50 μg/ml) were used as negative & positive controls. Western blot analysis & staining with anti-phospho-MLC, anti-total-MLC, & anti-beta actin antibodies was then performed. Phospho-MLC, total-MLC, & beta actin were all obtained with their appropriate protein size bands. The relative Phospho-MLC intensity (%) was expressed as [(Phospho-MLC/total-MLC)drug treated/ (Phospho-MLC/total-MLC)cell only x 100%]. The Mean±SEM values for at least three independent experiments along with the representative western blot were performed. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0145109), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-74340_mouse-monoclonal-actin-antibody-mabgea-310202415533829.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0012.jpg "Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP2-25142) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0010.jpg)
![Flow Cytometry: Actin Antibody (5J11) [NBP2-25142] - analysis of HeLa cells using mouse Monoclonal Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Flow-Cytometry-NBP2-25142-img0008.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of HeLa, A431, K562, MCF-7, NIH-3T3, C6, and Rat-2 cell lines.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0007.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 ug/mL mouse anti-Actin monoclonal (NBP2-25142, Novus Biologicals), followed by 1:2000 dilution of HRP- conjugated secondary antibody, donkey anti-mouse IgG (HAF018, R&D Systems).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0009.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of tissue and cell lysates probed with mouse mAb against actin, NBP2-25142, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells, in green.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - HeLa cells stained with NBP2-25142 (green) and also with the chicken polyclonal antibody to Vimentin (NB300-223, red). The Actin antibody stains the submembranous Actin rich cytoskeleton and also stress fibers, bundles of Actin associated with adhesion sites. The Vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0003.jpg)
![Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Western-Blot-NB120-11003-img0004.jpg "Western Blot: pan Actin Antibody (AC-40) [NB120-11003] - Cell line lysates lanes: 1. HeLa, 2. JURKAT, 3. COS7, 4. NIH-3T3, 5. PC-12, 6. RAT2, 7. CHO, 8. MDBK, 9. MDCK.")
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - CFB cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with Monoclonal Anti-Actin produced in mouse clone No. AC-40. The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - conserved C-terminus specific Monoclonal staining of human tongue frozen section with Monoclonal Anti-Actin](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: pan Actin Antibody (AC-40) [NB120-11003] - Rat2 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:250 Monoclonal Anti-Actin Clone: AC-40. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/pan-Actin-Antibody-AC-40-Immunocytochemistry-Immunofluorescence-NB120-11003-img0005.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0011.jpg "Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Endometrium muscle tissue lysate using pan Actin Antibody (HHF35).")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed paraffin-embedded human Rhabdomyosarcoma stained with muscle specific actin monoclonal antibody (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0008.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Analysis of Skeletal muscle tissue lysate using pan Actin Antibody (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP2-34230-img0010.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Formalin-fixed, paraffin-embedded human leiomyosarcoma (10X) stained with muscle specific actin MAb (HHF35)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP2-34230-img0004.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Simple Western lane view shows a specific band for pan Actin in 0.2 mg/ml of Muscle (Sk) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0005.jpg)
![Simple Western: Actin (Muscle Specific) Antibody (HHF35) [NBP2-34230] - Electropherogram image of the corresponding Simple Western lane. pan Actin antibody was used at 10 ug/ml dilution of Muscle (Sk) lysates(s) respectively.](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Simple-Western-NBP2-34230-img0007.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0008.jpg "Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates with Rabbit anti-Actin antibody at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)Lane 2: A431 cell lysate (10 ug/Lane)Lane 3: PC-12 cell lysate (10 ug/Lane)Lane 4: Mouse brain tissue lysate (20 ug/Lane)Lane 5: Rat kidney tissue lysate (20 ug/Lane)Predicted band size: 42 kDaObserved band size: 42 kDaExposure time: 30 seconds;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-80) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0006.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Actin with Rabbit anti-Actin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0009.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0005.jpg)
![Western Blot: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Western-Blot-NBP2-67907-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (JJ09-29) [NBP2-67907] - Staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunocytochemistry-Immunofluorescence-NBP2-67907-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (JJ09-29) [NBP2-67907] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Actin-Antibody-JJ09-29-Immunohistochemistry-Paraffin-NBP2-67907-img0004.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Western-Blot-NBP2-45274-img0006.jpg "Western Blot: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - hSKM cell lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953)")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Formalin-fixed paraffin-embedded rat stomach stained with muscle specific actin monoclonal antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0007.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0004.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-MSA-953-Immunohistochemistry-Paraffin-NBP2-45274-img0005.jpg)
![Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - Flow (Intracellular): Actin (Muscle Specific) Antibody (MSA/953) [NBP2-45274] - An intracellular stain was performed on HepG2 cells with pan Actin [MSA/953] Antibody NBP2-47662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin format of this antibody.](http://images.novusbio.com/fullsize/nbp2-45274_mouse-monoclonal-pan-actin-antibody-msa-953-17720249362611.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free [NBP2-47663] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/pan-Actin-Antibody-HHF35-+-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47663-img0005.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free [NBP2-47663] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free [NBP2-47663] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/pan-Actin-Antibody-HHF35-+-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47663-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free [NBP2-47663] - Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/pan-Actin-Antibody-HHF35-+-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47663-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free [NBP2-47663] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/pan-Actin-Antibody-HHF35-+-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47663-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) - Azide and BSA Free [NBP2-47663] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/pan-Actin-Antibody-HHF35-+-MSA-953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47663-img0004.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [NBP2-45275] - Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-+-MSA-953-Immunohistochemistry-Paraffin-NBP2-45275-img0005.jpg "Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [NBP2-45275] - Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [NBP2-45275] - Human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-+-MSA-953-Immunohistochemistry-Paraffin-NBP2-45275-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [NBP2-45275] - Human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-+-MSA-953-Immunohistochemistry-Paraffin-NBP2-45275-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [NBP2-45275] - Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-+-MSA-953-Immunohistochemistry-Paraffin-NBP2-45275-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [NBP2-45275] - Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-+-MSA-953-Immunohistochemistry-Paraffin-NBP2-45275-img0004.jpg)
![Western Blot: Actin Antibody (3U6B8) [NBP3-16100] - Western blot analysis of extracts of various cell lines, using Actin Rabbit mAb (NBP3-16100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Actin-Antibody-3U6B8-Western-Blot-NBP3-16100-img0006.jpg "Western Blot: Actin Antibody (3U6B8) [NBP3-16100] - Western blot analysis of extracts of various cell lines, using Actin Rabbit mAb (NBP3-16100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] - Immunofluorescence analysis of mouse skeletal muscle using Actin Rabbit mAb (NBP3-16100) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Actin-Antibody-3U6B8-Immunohistochemistry-NBP3-16100-img0005.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (3U6B8) [NBP3-16100] - Immunohistochemistry of paraffin-embedded rat lung using Actin Rabbit mAb (NBP3-16100) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Actin-Antibody-3U6B8-Immunohistochemistry-Paraffin-NBP3-16100-img0001.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (3U6B8) [NBP3-16100] - Immunohistochemistry of paraffin-embedded human appendix using Actin Rabbit mAb (NBP3-16100) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Actin-Antibody-3U6B8-Immunohistochemistry-Paraffin-NBP3-16100-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (3U6B8) [NBP3-16100] - Immunohistochemistry of paraffin-embedded mouse heart using Actin Rabbit mAb (NBP3-16100) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Actin-Antibody-3U6B8-Immunohistochemistry-Paraffin-NBP3-16100-img0003.jpg)
![Immunohistochemistry: Actin Antibody (3U6B8) [NBP3-16100] - Immunofluorescence analysis of rat skeletal muscle using Actin Rabbit mAb (NBP3-16100) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Actin-Antibody-3U6B8-Immunohistochemistry-NBP3-16100-img0004.jpg)
![Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP3-09165] - Analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle Lane 2: murine heart Lane 3: HeLa Lane 4: HUVEC](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Western-Blot-NBP3-09165-img0001.jpg "Western Blot: Actin (Muscle Specific) Antibody (HHF35) [NBP3-09165] - Analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle Lane 2: murine heart Lane 3: HeLa Lane 4: HUVEC")
![Immunohistochemistry-Paraffin: Actin (Muscle Specific) Antibody (HHF35) [NBP3-09165] - Staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35).](http://images.novusbio.com/fullsize/Actin-Muscle-Specific-Antibody-HHF35-Immunohistochemistry-Paraffin-NBP3-09165-img0002.jpg)
![Western Blot: Actin Antibody [NBP2-98923] - Lane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa](http://images.novusbio.com/fullsize/Actin-Antibody-Western-Blot-NBP2-98923-img0006.jpg "Western Blot: Actin Antibody [NBP2-98923] - Lane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa")
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0005.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0002.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in human skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0003.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody [NBP2-98923] - Immunochemical staining Actin in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Actin-Antibody-Immunohistochemistry-Paraffin-NBP2-98923-img0004.jpg)
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0005.jpg "Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Striated muscle from human tongue immunostained with 3B3 (1250).")
![Immunohistochemistry-Paraffin: Actin Antibody (3B3) [NBP1-97723] - Paraffin sections of human striated skeletal muscle.](http://images.novusbio.com/fullsize/Actin-Alpha-1-Skeletal-Muscle-Antibody-3B3-Immunohistochemistry-Paraffin-NBP1-97723-img0003.jpg)