![Immunohistochemistry-Paraffin: ACTH Antibody (SPM333) [NBP2-32911] - Formalin-fixed, paraffin-embedded human Pituitary stained with ACTH Antibody (SPM333).](http://images.novusbio.com/fullsize/ACTH-Antibody-SPM333-Immunohistochemistry-Paraffin-NBP2-32911-img0001.jpg "Immunohistochemistry-Paraffin: ACTH Antibody (SPM333) [NBP2-32911] - Formalin-fixed, paraffin-embedded human Pituitary stained with ACTH Antibody (SPM333).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clone | SPM333 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34761) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Synthetic peptide corresponding to aa1-24 of human ACTH (Uniprot: P01189) |
| Epitope | aa1-24 |
| Localization | Cytoplasmic |
| Marker | Adrenal Marker |
| Specificity | ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This monoclonal antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. ' |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POMC |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin-fixed tissues. Optimal dilution for a specific application should be determined. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-32911 | Applications | Species |
|---|---|---|
| Duan S, Sawyer TW, Sontz RA et al. GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming Cellular and molecular gastroenterology and hepatology 2022-07-11 [PMID: 35835391] (IHC-P, Mouse) | IHC-P | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for ACTH Antibody (NBP2-32911)Find related products by research area.
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