Orthogonal Strategies: Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining in human small intestine and pancreas tissues using anti-ACSL5 antibody. Corresponding ACSL5 RNA-seq data are presented ...read more
Western Blot: ACSL5 Antibody [NBP2-31995] - Analysis in control (vector only transfected HEK293T lysate) and ACSL5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ACSL5 Antibody [NBP2-31995] - Staining of human cell line U-251 MG shows localization to nucleoli & mitochondria. Antibody staining is shown in green.
Western Blot: ACSL5 Antibody [NBP2-31995] - Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining of human small intestine shows high expression.
This antibody was developed against a recombinant protein corresponding to amino acids: VHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACSL5
Purity
Immunogen affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for ACSL5 Antibody
ACS2
ACS5FACL5 for fatty acid coenzyme A ligase 5
acyl-CoA synthetase long-chain family member 5
EC 6.2.1
EC 6.2.1.3
FACL5fatty acid coenzyme A ligase 5
fatty-acid-Coenzyme A ligase, long-chain 5
LACS 5
Long-chain acyl-CoA synthetase 5
long-chain fatty acid coenzyme A ligase 5
long-chain-fatty-acid--CoA ligase 5
Background
Long-chain-fatty-acid--CoA ligase 5, or ACSL5, is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. These proteins play an important role in both synthesis of cellular lipids and degradation via beta-oxidation. Like other isozymes of this family, ALCS5 converts free long-chain fatty acids into fatty acyl-CoA esters. Specifically, ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage.
This isozyme is highly expressed in uterus and spleen, and in trace amounts are found in normal brain. It also has significantly increased expression in malignant gliomas, where the protein functions in mediating fatty acid-induced glioma cell growth. Therefore, ALCS5 is thought to have a key role in the survival of glioma cells and may be a potential target for novel cancer treatments. Three transcript variants encoding two different isoforms have been found for this gene.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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