ACSL5 Antibodies
We offer ACSL5 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each ACSL5 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ACSL5 Antibodies can be used in a variety of model species: Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish. Use the list below to choose the ACSL5 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ACSL5 polyclonal antibodies and browse our ACSL5 monoclonal antibody catalog.
Alternate Names for ACSL5 Antibodies
anti-ACSL5 antibody, anti-ACS2 antibody, anti-ACS5FACL5 for fatty acid coenzyme A ligase 5 antibody, anti-acyl-CoA synthetase long-chain family member 5 antibody, anti-EC 6.2.1 antibody, anti-EC 6.2.1.3 antibody, anti-FACL5fatty acid coenzyme A ligase 5 antibody, anti-fatty-acid-Coenzyme A ligase, long-chain 5 antibody, anti-LACS 5 antibody, anti-Long-chain acyl-CoA synthetase 5 antibody, anti-long-chain fatty acid coenzyme A ligase 5 antibody, anti-long-chain-fatty-acid--CoA ligase 5 antibody
10 Results for "acsl5" in Primary Antibodies
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Western-Blot-H00051703-M01-img0012.jpg "Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - ACSL5 monoclonal antibody (M01), clone 5H8 Analysis of ACSL5 expression in HepG2.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Immunohistochemistry-Paraffin-H00051703-M01-img0009.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody (M01), clone 5H8.Lane 1: ACSL5 transfected lysate(82.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Western-Blot-H00051703-M01-img0013.jpg)
![Immunoprecipitation: ACSL5 Antibody (5H8) [H00051703-M01] - Analysis of ACSL5 transfected lysate using anti-ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Immunoprecipitation-H00051703-M01-img0010.jpg)
![Sandwich ELISA: ACSL5 Antibody (5H8) [H00051703-M01] - Detection limit for recombinant GST tagged ACSL5 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ACSL5-Antibody-5H8-Sandwich-ELISA-H00051703-M01-img0011.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Representative western blotting (n = 3) for U87Empty, U87WT, and U87Mut cell protein extracts. The membranes were blotted with ACSL5 (H00051703-M01). The relative quantification of the bands is shown under the line. Blot was stripped and reprobed with anti-beta actin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35574033/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00051703-m01_mouse-monoclonal-acsl5-antibody-5h8-25520231539511.jpg)
![Western Blot: ACSL5 Antibody (5H8) [H00051703-M01] - Effects of IGF1R localization over cell metabolism. (A) U87Empty, U87WT, & U87Mut cells were cultured 24 h in serum-free medium or with 50-nM IGF1 stimulation (IGF1). To test the specificity of the response, pre-incubation (1 h) with 0.5 μM OSI906 was also performed (IGF1+ OSI). (A, B) GLUT1 mRNA expression was calculated by rqPCR by the relative quantitation method. (A) Values are presented as fold change compared to control conditions (serum-free). (B) IGF1 stimuli comparison between cell lines. The results are presented as fold change due to IGF1 stimulation over basal conditions (serum-free). Values are expressed as mean ± SD of three independent experiments performed in triplicates (*p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001, ANOVA, Tukey’s post-test). (C) LDH enzyme activity was measured & normalized to DNA content (mg). The results are expressed as mean ± SD of three independent experiments (**p < 0.005, ANOVA, Tukey’s post-test). (D) Representative western blotting (n = 3) for U87Empty, U87WT, & U87Mut cell protein extracts. The membranes were blotted with anti-pPDC (line 1), anti-FAS (line 3), anti-ACSL5 (line 5), & anti-pS6K (line 7). Each blot was stripped & reprobed with anti-beta actin (lines 2, 4, & 6) or antitotal-S6K (line 8). The relative quantification of the bands is shown under each line. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35574033), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00051703-m01_mouse-monoclonal-acsl5-antibody-5h8-310202415482271.jpg)
![Western Blot: ACSL5 Antibody [NBP1-59645] - Detection of ACSL5 in rat lysate. Image provided by Dr. Eric Klett of UNC School of Medicine.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP1-59645-img0004.jpg "Western Blot: ACSL5 Antibody [NBP1-59645] - Detection of ACSL5 in rat lysate. Image provided by Dr. Eric Klett of UNC School of Medicine.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP1-59645] - IHC analysis of human skeletal muscle tissue. Antibody concentration of 4 - 8ug/mL.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP1-59645-img0003.jpg)
![Western Blot: ACSL5 Antibody [NBP1-59645] - Human placenta tissue lysate. Antibody at a concentration of 1 ug/mL.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP1-59645-img0001.jpg)
![Simple Western: ACSL5 Antibody [NBP1-59645] - Simple Western analysis of mouse liver lysate. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Simple-Western-NBP1-59645-img0007.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining in human small intestine and pancreas tissues using anti-ACSL5 antibody. Corresponding ACSL5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-31995-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining in human small intestine and pancreas tissues using anti-ACSL5 antibody. Corresponding ACSL5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ACSL5 Antibody [NBP2-31995] - Analysis in control (vector only transfected HEK293T lysate) and ACSL5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP2-31995-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: ACSL5 Antibody [NBP2-31995] - Staining of human cell line U-251 MG shows localization to nucleoli & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31995-img0009.jpg)
![Western Blot: ACSL5 Antibody [NBP2-31995] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NBP2-31995-img0008.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-31995-img0010.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-31995] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-31995-img0011.jpg)
![Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Western-Blot-NBP2-52899-img0006.jpg "Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human small intestine shows very strong cytoplasmic and membrane immunoreactivity in the glandular cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon (colorectal cancer) shows moderate membrane positivity in cancer cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon shows strong positivity in the intestinal glands.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0002.jpg)
![Immunohistochemistry: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human duodenum shows strong cytoplasmic and membrane immunoreactivity in the glandular cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-NBP2-52899-img0003.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ACSL5 Antibody [NB100-2918] - Staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NB100-2918-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NB100-2918] - Staining of human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NB100-2918-img0004.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-298202414333655.jpg "Immunohistochemistry: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31996) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D3-R) [NBP3-31996] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31996) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 65 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31996) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31996_mouse-acsl5-mab-a9d3-r-392024848509.jpg)
![Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-29820241492626.jpg "Immunohistochemistry: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31995) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ACSL5 Antibody (A9D4-R) [NBP3-31995] - Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (NBP3-31995) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31995) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31995_mouse-acsl5-mab-a9d4-r-3920248453515.jpg)
![Western Blot: ACSL5 Antibody [NBP3-35416] - Western blot analysis of various lysates using ACSL5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35416_rabbit-acsl5-pab-1012202411545513.jpg "Western Blot: ACSL5 Antibody [NBP3-35416] - Western blot analysis of various lysates using ACSL5 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-97725] - ACSL5 in human liver with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NBP2-97725-img0001.jpg "Immunohistochemistry-Paraffin: ACSL5 Antibody [NBP2-97725] - ACSL5 in human liver with rabbit polyclonal antibody at 1:100 dilution.")