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ACOT8 Antibody

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Western Blot: ACOT8 Antibody [NBP1-32452] - Rat tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with ACOT8 antibody diluted by 1:1000.
Western Blot: ACOT8 Antibody [NBP1-32452] - Sample(30 ug whole cell lysate)A:MOLT4 B:Raji 10% SDS PAGE, antibody diluted at 1:1000.
Western Blot: ACOT8 Antibody [NBP1-32452] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ACOT8 antibody diluted by 1:1000.
Western Blot: ACOT8 Antibody [NBP1-32452] - Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with ACOT8 antibody diluted by 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ACOT8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ACOT8. The exact sequence is proprietary.
Localization
Peroxisome
Predicted Species
Monkey (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACOT8
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (97%), Bovine (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for ACOT8 Antibody

  • ACTEIII
  • acyl-CoA thioesterase 8HNAACTE
  • Choloyl-coenzyme A thioesterase
  • EC 3.1.2.27
  • hACTEIII
  • HIV-Nef-associated acyl-CoA thioesterase
  • hTEcholoyl-CoA hydrolase
  • long-chain fatty-acyl-CoA hydrolase
  • palmitoyl-CoA hydrolase
  • peroxisomal acyl-CoA thioesterase 1
  • peroxisomal acyl-CoA thioesterase
  • Peroxisomal acyl-coenzyme A thioester hydrolase 1
  • Peroxisomal long-chain acyl-CoA thioesterase 1
  • PTE-1
  • PTE1HIV-Nef associated acyl-CoA thioesterase
  • PTE2
  • PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8
  • Thioesterase II
  • thioesterase III

Background

The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACOT8