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ACOT11 Antibody

Images

 
Western Blot: ACOT11 Antibody [NBP2-58936] - Western blot analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: ACOT11 Antibody [NBP2-58936] - Staining of human cell line A-431 shows localization to cytosol.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ACOT11 Antibody [NBP2-58936] - Staining in human duodenum and pancreas tissues using anti-ACOT11 antibody. Corresponding ACOT11 RNA-seq data are presented for ...read more
Immunohistochemistry-Paraffin: ACOT11 Antibody [NBP2-58936] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: ACOT11 Antibody [NBP2-58936] - Staining of human pancreas shows low expression as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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ACOT11 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FLLLSDLRQRPEWDKHYRSVELVQQVDEDDAIYHVTSPALGGHTKPQDFVILASRRKPCDNGDPYVIALRSVTLPTHRETPEYRRGE
Predicted Species
Mouse (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACOT11
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ACOT11 Recombinant Protein Antigen (NBP2-58936PEP)

Reactivity Notes

Rat 89%

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ACOT11 Antibody

  • Acyl-CoA thioester hydrolase 11
  • acyl-CoA thioesterase 11BFIT1
  • Adipose-associated thioesterase
  • BFIT2
  • BFITDKFZp667O1916
  • Brown fat-inducible thioesterase
  • EC 3.1.2.-
  • EC 3.1.2.1
  • KIAA0707acyl-coenzyme A thioesterase 11
  • STARD14
  • START domain containing 14
  • THEAStAR-related lipid transfer (START) domain containing 14
  • THEM1
  • thioesterase superfamily member 1
  • thioesterase, adipose associated

Background

ACOT11 (Acyl-CoA thioesterase11), also known as BFIT, is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of this protein in mouse has been associated with obesity and alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ACOT11 Antibody (NBP2-58936) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ACOT11