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ACD Antibody (1D8-1B6)

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 (1 Review)
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Western Blot: ACD Antibody (1D8-1B6) [H00065057-M02] - Western Blot analysis of ACD expression in IMR-32 ( Cat # L008V1 ).
Western Blot: ACD Antibody (1D8-1B6) [H00065057-M02] - Western Blot analysis of ACD expression in IMR-32 ( Cat # L008V1 ).
Immunocytochemistry/ Immunofluorescence: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of monoclonal antibody to ACD on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 2 ug/ml.
ELISA: ACD Antibody (1D8-1B6) [H00065057-M02] - Detection limit for recombinant GST tagged ACD is 0.3 ng/ml as a capture antibody.
Immunoprecipitation: ACD Antibody (1D8-1B6) [H00065057-M02] - Analysis of ACD transfected lysate using anti-ACD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACD MaxPab rabbit polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP, ChIP
Clone
1D8-1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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ACD Antibody (1D8-1B6) Summary

Immunogen
ACD (AAH16904, 1 a.a. ~ 544 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPGRCQSDAAMRVNGPASRAPAGWTSGSLHTGPRAGRPRAQARGVRGRGLLLRPRPAKELPLPRKGGAWAPAGNPGPLHPLGVAVGMAGSGRLVLRPWIRELILGSETPSSPRAGQLLEVLQDAEAAVAGPSHAPDTSDVGATLLVSDGTHSVRCLVTREALDTSDWEEKEFGFRGTEGRLLLLQDCGVHVQVAEGGAPAEFYLQVDRFSLLPTEQPRLRVPGCNQDLDVQKKLYDCLEEHLSESTSSNAGLSLSQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSSMLCISENDQLILSSLGPCQRTQGPELPPPDPALQDLSLTLIASPPSSPSSSGTPALPGHMSSEESGTSISLLPALSLAAPDPGQRSSSQPSPAICSAPATLTPRSPHASRTPSSPLQSCTPSLSPRSHVPSPHQALVTRPQKPSLEFKEFVGLPCKNRPPFPRTGATRGAQEPCSVWEPPKRHRDGSAFQYEYEPPCTSLCARVQAARLPPQLMAWALHFLMDAQPGSEPTPM
Specificity
ACD - adrenocortical dysplasia homolog (mouse)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACD
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Use in chromatin immunoprecipitation reported in scientific literature (PMID 25233904)
Reviewed Applications
Read 1 Review rated 3
using
H00065057-M02 in the following applications:
Publications
Read Publications using
H00065057-M02 in the following applications:

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ACD Antibody (1D8-1B6)

  • adrenocortical dysplasia homolog (mouse)
  • Pip1
  • PIP1Tint1
  • POT1 and TIN2 organizing protein
  • POT1 and TIN2-interacting protein
  • Ptop
  • PTOPTpp1
  • TIN2 interacting protein 1
  • TINT1adrenocortical dysplasia protein homolog
  • TPP1

Background

This gene encodes a protein that is involved in telomere function. This protein is one of six core proteins in the telosome/shelterin telomeric complex, which functions to maintain telomere length and to protect telomere ends. Through its interaction with other components, this protein plays a key role in the assembly and stabilization of this complex, and it mediates the access of telomerase to the telomere. Multiple transcript variants encoding different isoforms have been found for this gene. This gene, which is also referred to as TPP1, is distinct from the unrelated TPP1 gene on chromosome 11, which encodes tripeptidyl-peptidase I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00065057-M02
ACD Antibody (1D8-1B6)
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IP, ChIP

Publications for ACD Antibody (H00065057-M02)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Chemotaxis.


Filter By Application
Chemotaxis
(1)
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Human
(2)
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Showing Publications 1 - 8 of 8.
Publications using H00065057-M02 Applications Species
Sandhu R, Wei D, Sharma M et al. An N-terminal Flag-tag impairs TPP1 regulation of telomerase function Biochem Biophys Res Commun 2019-03-20 [PMID: 30885434]
Zanini IM, Soneson C, Lorenzi LE, Azzalin CM. Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion. J Cell Sci 2017-02-15 [PMID: 28062851]
Frank AK, Tran DC, Qu RW et al. The Shelterin TIN2 Subunit Mediates Recruitment of Telomerase to Telomeres. PLoS Genet. 2015-07-31 [PMID: 26230315]
Kocak H, Ballew BJ, Bisht K et al. Hoyeraal-Hreidarsson syndrome caused by a germline mutation in the TEL patch of the telomere protein TPP1. Genes Dev. 2014-10-01 [PMID: 25233904] (Chemotaxis, Human) Chemotaxis Human
Nandakumar J, Bell CF, Weidenfeld I et al. The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity. Nature. 2012-12-13 [PMID: 23103865] (Human) Human
Lakamp AS, Ouellette MM. A ssDNA Aptamer That Blocks the Function of the Anti-FLAG M2 Antibody. J Nucleic Acids;2011:720798. 2011-01-01 [PMID: 22013507]
Choi KH, Farrell AS, Lakamp AS et al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res. 2011-11-01 [PMID: 21852327]

Details:
Tin2 (IMG-282) & POT1 (IMG-5343A). EMSA: HeLa cell line transfected with or without Flag-Tin2, Fig 4C.
Abreu E, Aritonovska E, Reichenbach P et al. TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo. Mol Cell Biol. 2010-06-01 [PMID: 20404094]

Review for ACD Antibody (H00065057-M02) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00065057-M02:
Filter by Applications
WB
(1)
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Human
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Images Ratings Applications Species Date Details
Western Blot ACD H00065057-M02
Enlarge 		3
reviewed by:
Verified Customer
 WB Human 09/01/2015
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Summary

ApplicationWestern Blot
Sample Testedhuman lung homogenates
SpeciesHuman
LotD5201-S1

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Western Blot ACD H00065057-M02
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 Verified Customer
09/01/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol ACD
Entrez
OMIM
Uniprot