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POR/Cytochrome P450 Reductase Antibody (OTI3F10)

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Western Blot: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 Reductase (Right lane) cDNA for 48 hrs ...read more
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 Reductase.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Flow Cytometry: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ...read more
Immunocytochemistry/ Immunofluorescence: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of HT29 cells using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Cytochrome P450 Reductase mouse monoclonal ...read more
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 Reductase mouse monoclonal ...read more
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 Reductase mouse monoclonal ...read more
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Human endometrium tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Human Ovary tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.
Immunohistochemistry-Paraffin: POR/Cytochrome P450 Reductase Antibody (3F10) [NBP1-47914] - Staining of paraffin-embedded Human pancreas tissue using anti-Cytochrome P450 Reductase mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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POR/Cytochrome P450 Reductase Antibody (OTI3F10) Summary

Immunogen
Full length human recombinant protein of human POR (NP_000932) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens P450 (cytochrome) oxidoreductase (POR).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
Theoretical MW
76.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POR/Cytochrome P450 Reductase Antibody (OTI3F10)

  • CPR
  • CYPOR
  • CYPORFLJ26468
  • Cytochrome P450 Reductase
  • DKFZp686G04235
  • EC 1.6.2.4
  • NADPH--cytochrome P450 reductase
  • NADPH-dependent cytochrome P450 reductase
  • P450 (cytochrome) oxidoreductase
  • P450R
  • POR

Background

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol POR
Entrez