| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73129 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73130 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4338-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4338-SP | |||
![Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NB500-178-img0005.jpg "Western Blot: NUMB Antibody [NB500-178] - Detection of NUMB isoforms 1 and 2 in A431 whole cell lysate (20 ug) using 0.5 ug/ml of NB500-178. ECL detection: 30 seconds.")
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: NUMB Antibody [NB500-178] - The NUMB antibody was tested in HepG2 cells at a 1:500 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Numb Antibody [NB500-178] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Numb Antibody (NB500-178) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Numb-Antibody-Immunocytochemistry-Immunofluorescence-NB500-178-img0007.jpg)
![Simple Western: Numb Antibody [NB500-178] - Simple Western lane view shows a specific band for NUMB in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Numb-Antibody-Simple-Western-NB500-178-img0006.jpg)
![Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test](http://images.novusbio.com/fullsize/NUMB-Antibody-Western-Blot-NBP2-27181-img0004.jpg "Western Blot: NUMB Antibody [NBP2-27181] - Analysis of mouse brain lysate in the 1) absence and 2) presence of immunizing peptide using NUMB antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody (20301) and PicoTect ECL substrate solution were used for this test")
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human adrenal tissue stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0006.jpg)
![Immunohistochemistry-Paraffin: NUMB Antibody [NBP2-27181] - Formalin-fixed, paraffin-embedded human spleen stained with NUMB antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/NUMB-Antibody-Immunohistochemistry-Paraffin-NBP2-27181-img0005.jpg)
![Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Western-Blot-NBP2-45877-img0017.jpg "Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .")
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0016.jpg)
![Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Western-Blot-NBP2-45877-img0018.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0001.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lung tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0002.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0003.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0004.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0005.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0006.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0007.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0008.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0009.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0010.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human prostate tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0011.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0012.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0013.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0014.jpg)
![Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/Numb-Antibody-4F3-Immunohistochemistry-NBP2-45877-img0015.jpg)
![Western Blot: Numb Antibody (1B5) [NBP2-46284] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB.](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Western-Blot-NBP2-46284-img0008.jpg "Western Blot: Numb Antibody (1B5) [NBP2-46284] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB.")
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0007.jpg)
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0001.jpg)
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0002.jpg)
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human embryonic liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0003.jpg)
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0004.jpg)
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0005.jpg)
![Immunohistochemistry: Numb Antibody (1B5) [NBP2-46284] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Numb-Antibody-1B5-Immunohistochemistry-NBP2-46284-img0006.jpg)
![SDS-Page: Recombinant Human Numb Protein [H00008650-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00008650-P01-1.jpg "SDS-Page: Recombinant Human Numb Protein [H00008650-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Western-Blot-NBP3-16738-img0001.jpg "Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Numb antibody (NBP3-16738). Western blot was performed from the immunoprecipitate using Numb (NBP3-16738) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Immunoprecipitation-NBP3-16738-img0002.jpg)
![Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Western-Blot-NBP2-98717-img0002.jpg "Western Blot: Numb Antibody [NBP2-98717] - Anti-Numb rabbit polyclonal antibody at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 70 kDa")
![Immunoprecipitation: Numb Antibody [NBP2-98717] - Numb was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 ul anti-Numb rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-Numb rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 95 kDa](http://images.novusbio.com/fullsize/Numb-Antibody-Immunoprecipitation-NBP2-98717-img0001.jpg)
![Western Blot: NUMB Overexpression Lysate (Adult Normal) [NBL1-13870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUMB.](http://images.novusbio.com/fullsize/NUMB-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13870-img0002.jpg "Western Blot: NUMB Overexpression Lysate (Adult Normal) [NBL1-13870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NUMB.")