Home » Numb » Numb Antibodies » Numb Antibody (OTI4F3)
Novus Biologicals products are now on bio-techne.com

Numb Antibody (OTI4F3)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .
Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB .
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lymphoma tissue.
Western Blot: Numb Antibody (4F3) [NBP2-45877] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: ...read more
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human lung tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human kidney tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human prostate tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human pancreas tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human breast tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human thyroid tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human lung tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human tonsil tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Adenocarcinoma of Human breast tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Human Kidney tissue.
Immunohistochemistry: Numb Antibody (4F3) [NBP2-45877] - Analysis of Carcinoma of Human bladder tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73129AF350
Alexa Fluor 405
NBP2-73129AF405
Alexa Fluor 488
NBP2-73129AF488
Alexa Fluor 532
NBP2-73129AF532
Alexa Fluor 594
NBP2-73129AF594
Alexa Fluor 647
NBP2-73129AF647
Alexa Fluor 700
NBP2-73129AF700
Alexa Fluor 750
NBP2-73129AF750
Allophycocyanin
NBP2-73129APC
Biotin
NBP2-73129B
CoraFluor 1
NBP2-73129CL1
DyLight 350
NBP2-73129UV
DyLight 405
NBP2-73129V
DyLight 488
NBP2-73129G
DyLight 550
NBP2-73129R
DyLight 594
NBP2-73129DL594
DyLight 650
NBP2-73129C
DyLight 680
NBP2-73129FR
DyLight 755
NBP2-73129IR
FITC
NBP2-73129F
HRP
NBP2-73129H
Janelia Fluor 525
NBP2-73129JF525
Janelia Fluor 549
NBP2-73129JF549
Janelia Fluor 585
NBP2-73129JF585
Janelia Fluor 635
NBP2-73129JF635
Janelia Fluor 646
NBP2-73129JF646
Janelia Fluor 669
NBP2-73129JF669
PE
NBP2-73129PE
PerCP
NBP2-73129PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-73129
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Numb Antibody (OTI4F3) Summary

Immunogen
Full length human recombinant protein of human NUMB(NP_003735) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NUMB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
69.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Numb Antibody (OTI4F3)

  • h-Numb
  • mnb
  • m-numb
  • numb (Drosophila) homolog
  • numb homolog (Drosophila)
  • Numb
  • protein numb homolog
  • Protein S171
  • S171

Background

Numb is a transmembrane signaling receptor that is activated by the DSL family of ligands. Studies suggestthat mammalian Numb plays a role in progenitor cell fate in neurogenesis. Numb exerts its role on cell fate by antagonizing Notch signaling. The mammalian Numb gene undergoes alternative splicing to produce at least four functionally different Numb isoforms which regulate different processes. A loss of Numb expression has been demonstrated in breast cancer, non-small cell lung cancer and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NUMBL Antibody
NB100-88136
Immunoprecipitation NUMBL Antibody
Species: Hu
Applications: IP, WB (-)
Notch-1 Antibody
(mN1A) - BSA Free
NB100-78486
Immunohistochemistry-Paraffin Notch-1 Antibody (mN1A) - BSA Free
Knockout Validated Notch-1 Antibody (mN1A) - BSA Free
Flow Cytometry Notch-1 Antibody (mN1A) - BSA Free
(2 Reviews)
(10 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
Lnx1 Antibody
NBP1-80518
Western Blot Lnx1 Antibody
Western Blot Lnx1 Antibody
Species: Hu
Applications: WB
PARD3/Par3 Antibody
NBP1-88861
Immunohistochemistry-Paraffin PARD3/Par3 Antibody
Immunohistochemistry-Paraffin PARD3/Par3 Antibody
Immunohistochemistry-Paraffin PARD3/Par3 Antibody
(2 Reviews)
(10 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, Simple Western, WB
INSC Antibody
NBP1-90980
Immunohistochemistry INSC Antibody
Species: Hu
Applications: IHC, IHC-P
ZNF645 Antibody
NBP2-21037
Western Blot ZNF645 Antibody
Immunohistochemistry-Paraffin ZNF645 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
MUL1 Antibody
(5H6.2D5) - BSA Free
NBP2-31361
Western Blot MUL1 Antibody (5H6.2D5) - BSA Free
Immunohistochemistry-Paraffin MUL1 Antibody (5H6.2D5) - BSA Free
Immunohistochemistry-Paraffin MUL1 Antibody (5H6.2D5) - BSA Free
Species: Hu
Applications: IHC, IHC-P, WB
TRIM50 Antibody
NBP1-81988
Immunohistochemistry-Paraffin TRIM50 Antibody
Species: Hu
Applications: IHC, IHC-P
PTBP2 Antibody
NBP2-19997
Western Blot PTBP2 Antibody
Immunohistochemistry-Paraffin PTBP2 Antibody
Western Blot PTBP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
PTBP1 Antibody
(3H8)
H00005725-M01
Western Blot PTBP1 Antibody (3H8)
Western Blot PTBP1 Antibody (3H8)
Western Blot PTBP1 Antibody (3H8)
(4 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen beta Amyloid Antibody (MOAB-2) - BSA Free
Immunohistochemistry beta Amyloid Antibody (MOAB-2) - BSA Free
Immunocytochemistry/ Immunofluorescence beta Amyloid Antibody (MOAB-2) - BSA Free
(2 Reviews)
(70 Publications)
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
GPSM2 Antibody
NBP1-53125
Immunohistochemistry GPSM2 Antibody
Western Blot GPSM2 Antibody
Immunocytochemistry/ Immunofluorescence GPSM2 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NM23-H1 Antibody
NBP1-80992
Immunohistochemistry-Paraffin NM23-H1 Antibody
Immunohistochemistry-Paraffin NM23-H1 Antibody
Immunohistochemistry-Paraffin NM23-H1 Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PXR/NR1I2 Antibody
(H4417) [Unconjugated ...
PP-H4417-00
PXR/NR1I2 Antibody (H4417) [Unconjugated]
(2 Publications)
Species: Hu
Applications: DirELISA, IP, WB
PAR3/Thrombin
Receptor-like 2 Antib
NLS261
Immunohistochemistry-Paraffin PAR3/Thrombin Receptor-like 2 Antibody
Immunohistochemistry-Paraffin PAR3/Thrombin Receptor-like 2 Antibody
Immunohistochemistry-Paraffin PAR3/Thrombin Receptor-like 2 Antibody
Species: Hu
Applications: ICC, IHC, IHC-P, WB (-)
DAN/NBL1 Antibody
[Unconjugated]
AF755
DAN/NBL1 Antibody [Unconjugated]
(1 Publication)
Species: Mu
Applications: Block, WB
Numb Antibody
(OTI4F3)
NBP2-45877
Numb Antibody (OTI4F3)
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for Numb Antibody (NBP2-45877) (0)

There are no publications for Numb Antibody (NBP2-45877).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Numb Antibody (NBP2-45877) (0)

There are no reviews for Numb Antibody (NBP2-45877). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Numb Antibody (NBP2-45877) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Numb Products

Research Areas for Numb Antibody (NBP2-45877)

Find related products by research area.

Blogs on Numb

There are no specific blogs for Numb, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Numb Antibody (OTI4F3) and receive a gift card or discount.


Bioinformatics

Gene Symbol NUMB