Novus Biologicals products are now on bio-techne.com

FoxP3 Antibody - BSA Free

Images

 
(
Enlarge)
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, WB, Dual ISH-IHC, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

  • Loading...
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

FoxP3 Antibody - BSA Free Summary

Immunogen
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Flow Cytometry 1:8000
  • Immunoblotting reported in scientific literature (PMID 26485285)
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400. Use reported by customer review
  • Immunoprecipitation
  • Single Cell Western 100 ug/mL
  • Western Blot 1:2000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. SCW is validating using FoxP3-transfected HEK293T cells.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-39002 in the following applications:

Publications
Read Publications using
NB100-39002 in the following applications:

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Epitope affinity purified

Alternate Names for FoxP3 Antibody - BSA Free

  • AIID
  • AIIDMGC141961
  • anti-human foxp3
  • anti-mouse foxp3
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 ihc
  • foxp3 paraffin
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
DR2A00
Species: Hu
Applications: ELISA
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
10/21/2019
Application: IHC-P
Species: Bovine, Goat, Sheep

Verified Customer
10/06/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol FOXP3
Entrez
Uniprot