FoxP3 Antibody - BSA Free Summary
Immunogen |
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6] |
Localization |
Nuclear |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
FOXP3 |
Purity |
Epitope affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Dual RNAscope ISH-IHC
- Flow Cytometry 1:8000
- Immunoblotting reported in scientific literature (PMID 26485285)
- Immunocytochemistry/ Immunofluorescence 1:500
- Immunohistochemistry 1:400
- Immunohistochemistry-Paraffin 1:400. Use reported by customer review
- Immunoprecipitation
- Single Cell Western 100 ug/mL
- Western Blot 1:2000
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Application Notes |
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. SCW is validating using FoxP3-transfected HEK293T cells. |
Reviewed Applications |
Read 2 Reviews rated 4 using NB100-39002 in the following applications:
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Publications |
Read Publications using NB100-39002 in the following applications:
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Reactivity Notes
This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.05% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Epitope affinity purified |
Alternate Names for FoxP3 Antibody - BSA Free
Background
FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).
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Does FoxP3 antibodies comes in lyophilized form? ..show answer..
- FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
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What the theoretical molecular weight for FoxP3 antibodies? ..show answer..
- The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
-
What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues? ..show answer..
- The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
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What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue? ..show answer..
- For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
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What is the immunogen sequence of this FoxP3 antibody? ..show answer..
- A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.