![Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-230] - Analysis of human testis using SCP3 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunohistochemistry-Paraffin-NB300-230-img0003.jpg "Immunohistochemistry-Paraffin: SCP3/SYCP3 Antibody [NB300-230] - Analysis of human testis using SCP3 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining was observed. Staining was performed by Histowiz.")
| Immunogen | A synthetic peptide made to the N-terminal region of the mouse SCP3 protein. [UniProt# P70281] |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SYCP3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Western Blot usage reported in scientific literature (PMID: 27462424). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry ERMAP Antibody [Unconjugated]](https://images.novusbio.com/images2/ERMAP_AF4928_Flow_Cytometry_11957.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
IF | Mouse | 12/23/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for SCP3/SYCP3 Antibody (NB300-230)Find related products by research area.
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SCP3: A Key to Meiotic Recombination, Sterility and Cancer Synaptonemal Complex Protein 3 (SCP3), which is a protein present in the synaptonemal complex which is responsible for pairing, synapsis, and recombination of homologous chromosomes during meiosis. Meiosis, in basic terms, is where germ cells divide t... Read full blog post. |
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| Gene Symbol | SYCP3 |
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-230] - Senataxin is SUMOylated at the XY chromosomes in spermatocytes. Immunostaining for senataxin revealed its localization to the XY chromosomes as a diffuse cloud during the pachytene stage in spermatocytes of Setx+/+ mice. Some background staining was also observed on the autosomes. As expected, no senataxin was detected in Setx-/- spermatocytes. Dotted circle, XY chromosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/celldisc201525), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-230-img0004.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-230] - Senataxin is SUMOylated at the XY chromosomes in spermatocytes. Immunostaining for senataxin revealed its localization to the XY chromosomes as a diffuse cloud during the pachytene stage in spermatocytes of Setx+/+ mice. Some background staining was also observed on the autosomes. As expected, no senataxin was detected in Setx-/- spermatocytes. Dotted circle, XY chromosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/celldisc201525), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-230] - Mouse pachytene preparation (red). CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-230-img0002.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-230] - Mouse pachytene preparation (red). CDK2 staining, near teleomeres, is also present (green).")
![Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-230] - Senataxin is SUMOylated at the XY chromosomes in spermatocytes. Immunostaining for SUMO-1 in Setx+/+ and Setx-/- spermatocytes revealed a diffuse staining over the XY chromosomes in Setx+/+ spermatocytes. In contrast, staining was largely restricted to the axes of the XY chromosomes in Setx-/- spermatocytes. Dotted circle, XY chromosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/celldisc201525), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP3-SYCP3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-230-img0005.jpg "Immunocytochemistry/Immunofluorescence: SCP3/SYCP3 Antibody [NB300-230] - Senataxin is SUMOylated at the XY chromosomes in spermatocytes. Immunostaining for SUMO-1 in Setx+/+ and Setx-/- spermatocytes revealed a diffuse staining over the XY chromosomes in Setx+/+ spermatocytes. In contrast, staining was largely restricted to the axes of the XY chromosomes in Setx-/- spermatocytes. Dotted circle, XY chromosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/celldisc201525), licensed under a CC-BY license.")
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