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CTDSP1 Antibody (OTI1B6) - Azide and BSA Free

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Western Blot: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTDSP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Human lymph node tissue using anti-CTDSP1 mouse monoclonal antibody.
Flow Cytometry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Human prostate tissue using anti-CTDSP1 mouse monoclonal antibody.
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CTDSP1 mouse monoclonal antibody.
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CTDSP1 mouse monoclonal antibody.
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CTDSP1 mouse monoclonal antibody.
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CTDSP1 mouse monoclonal antibody.
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Human Kidney tissue using anti-CTDSP1 mouse monoclonal antibody.
Immunohistochemistry: CTDSP1 Antibody (OTI1B6) - Azide and BSA Free [NBP2-72110] - Staining of paraffin-embedded Human liver tissue using anti-CTDSP1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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CTDSP1 Antibody (OTI1B6) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human CTDSP1 (NP_067021) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CTDSP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CTDSP1 Antibody (OTI1B6) - Azide and BSA Free

  • carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
  • CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) smallphosphatase 1
  • NIF3
  • NLI-IF
  • NLIIFEC 3.1.3.16
  • NLI-interacting factor 3
  • SCP1Nuclear LIM interactor-interacting factor 3
  • Small CTD phosphatase 1
  • Small C-terminal domain phosphatase 1

Background

Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in theC-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase IItranscription, possibly by controlling the transition from initiation/capping to processive transcript elongation.Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronalcells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CTDSP1