![Western Blot: ZNF503 Antibody [NBP1-81889] - Analysis in human cell lines PC-3 and MCF-7 using anti-ZNF503 antibody. Corresponding ZNF503 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.](http://images.novusbio.com/fullsize/ZNF503-Antibody-Western-Blot-NBP1-81889-img0014.jpg "Western Blot: ZNF503 Antibody [NBP1-81889] - Analysis in human cell lines PC-3 and MCF-7 using anti-ZNF503 antibody. Corresponding ZNF503 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: SPSSKLSSVASNGGGAGGAGGGAAGDKDTKSGPLKLSDIGVEDKSSFKPYSKPGSDKKEPGGGGGGGGGGGGGGGGVSSEKSGFRVPSATCQPFTPRTGSPSSSASACSPGGMLSSAGGAPEGKDDKKDTDVG |
| Predicted Species | Mouse (92%), Rat (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ZNF503 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ZNF503 |
![Immunocytochemistry/Immunofluorescence: ZNF503 Antibody [NBP1-81889] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZNF503-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81889-img0007.jpg "Immunocytochemistry/Immunofluorescence: ZNF503 Antibody [NBP1-81889] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human tonsil shows only very low positivity in lymphoid cells.](http://images.novusbio.com/fullsize/ZNF503-Antibody-Immunohistochemistry-Paraffin-NBP1-81889-img0012.jpg "Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human tonsil shows only very low positivity in lymphoid cells.")
![Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/ZNF503-Antibody-Immunohistochemistry-Paraffin-NBP1-81889-img0009.jpg "Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.")
![Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ZNF503-Antibody-Immunohistochemistry-Paraffin-NBP1-81889-img0010.jpg "Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human prostate shows moderate nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human rectum shows moderate to strong nuclear positivity in smooth muscle cells, as well as weak positivity in glandular cells.](http://images.novusbio.com/fullsize/ZNF503-Antibody-Immunohistochemistry-Paraffin-NBP1-81889-img0011.jpg "Immunohistochemistry-Paraffin: ZNF503 Antibody [NBP1-81889] - Staining of human rectum shows moderate to strong nuclear positivity in smooth muscle cells, as well as weak positivity in glandular cells.")
![Simple Western Islet-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Islet1_AF1837_Simple_Western_21249.jpg)
![Western Blot Islet-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Islet1_AF1837_Western_Blot_21254.jpg)
![Immunocytochemistry Islet-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Islet1_AF1837_Immunocytochemistry__Immunofluorescence_17786.jpg)
![Western Blot DARPP-32 Antibody (375604) [Unconjugated]](https://images.novusbio.com/images2/DARPP-32_MAB4230_Western_Blot_6161.jpg)
![SDS-PAGE FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF-8_423-F8_332.jpg)
![Bioactivity FGF-8 [Unconjugated]](https://images.novusbio.com/images2/FGF8_423F8_1438.jpg)
![Cell Culture FGF-8 [Unconjugated]](https://images.novusbio.com/images/423-f8_recombinant-human-mouse-fgf-8b-protein-cell-culture-1052024145159..jpg)
![Bioactivity Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464SH_2840.jpg)
![SDS-PAGE Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images2/Sonic_Hedgehog_464-SH_341.jpg)
![SEC-MALS Sonic Hedgehog/Shh [Unconjugated]](https://images.novusbio.com/images/464-sh_recombinant-mouse-sonic-hedgehog-shh-c25ii-n-terminus-sec-mals-642023224324.jpg)
![Intracellular Staining by Flow Cytometry Reg1A Antibody (431202) [Unconjugated]](https://images.novusbio.com/images2/Reg1A_MAB4937_Flow_Cytometry_8512.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")