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ZFYVE1 Antibody

Images

 
Western Blot: ZFYVE1 Antibody [NBP1-84267] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: ZFYVE1 Antibody [NBP1-84267] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZFYVE1 Antibody [NBP1-84267] - Staining of human testis shows moderate positivity in cytoplasm granular in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ZFYVE1 Antibody [NBP1-84267] - Staining of human cerebellum shows moderate positivity in cytoplasm granular in Purkinje cells.
Immunohistochemistry-Paraffin: ZFYVE1 Antibody [NBP1-84267] - Staining of human kidney shows strong positivity in cytoplasm granular in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ZFYVE1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: IAPAYWRPNSQILSCNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERGWGPAPVRVCDNCYEARNVQLAVTEAQVDDEGGTLIARKVGEAVQNTLGAVVTAIDIPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHH
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZFYVE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
ZFYVE1 Protein (NBP1-84267PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ZFYVE1 Antibody

  • DFCP1
  • DFCP1phosphoinositide-binding protein SR3
  • Double FYVE-containing protein 1
  • KIAA1589tandem FYVE fingers-1 protein
  • SR3
  • TAFF1zinc finger FYVE domain-containing protein 1
  • Tandem FYVE fingers-1
  • zinc finger protein, subfamily 2A (FYVE domain containing), 1
  • zinc finger, FYVE domain containing 1
  • ZNFN2A1zinc finger protein, subfamily 2A, member 1

Background

The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate (PtdIns(3)P)-containing membranes. This gene encodes a protein which contains two zinc-binding FYVE domains in tandem. This protein displays a predominantly Golgi, endoplasmic reticulum and vesicular distribution. Alternatively spliced transcript variants have been found for this gene, and they encode two isoforms with different sizes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ZFYVE1