![Western Blot: Wnt-11 Antibody [NBP1-31406] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Western-Blot-NBP1-31406-img0018.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Wnt-11. The exact sequence is proprietary. |
| Localization | Secreted, extracellular space, extracellular matrix |
| Predicted Species | Bovine (98%), Rabbit (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | WNT11 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
![Wnt-2b Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Wnt-2b Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Wnt-11 Antibody (NBP1-31406)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31406-img0016.jpg "Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded mouse colon. WNT11 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunohistochemistry-Paraffin-NBP1-31406-img0012.jpg "Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded mouse colon. WNT11 antibody dilution: 1:500.")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Western-Blot-NBP1-31406-img0017.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - Analysis of HeLa, using NBP1-31406 at 1:200 dilution.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31406-img0008.jpg "Immunocytochemistry/Immunofluorescence: Wnt-11 Antibody [NBP1-31406] - Analysis of HeLa, using NBP1-31406 at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Wnt-11-Antibody-Immunohistochemistry-Paraffin-NBP1-31406-img0009.jpg "Immunohistochemistry-Paraffin: Wnt-11 Antibody [NBP1-31406] - Paraffin-embedded lung SCC xenograft, using antibody at 1:100 dilution.")
![Western Blot: Wnt-11 Antibody [NBP1-31406] -Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31406_rabbit-polyclonal-wnt-11-antibody-western-blot-7102024115010..jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] -Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31406_rabbit-polyclonal-wnt-11-antibody-91020242091422.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31406_rabbit-polyclonal-wnt-11-antibody-91020242013405.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Wnt-11 Antibody [NBP1-31406] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31406_rabbit-polyclonal-wnt-11-antibody-91020242021966.jpg "Western Blot: Wnt-11 Antibody [NBP1-31406] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt-11 antibody (NBP1-31406) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Bioactivity Wnt-5a [Unconjugated]](https://images.novusbio.com/images/protein/645-wn_recombinant-human-mouse-wnt-5a-protein-bioactivity-1612202094911.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/Wnt3a_5036WN_2880.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-3112023115329.jpg)
![Cell Culture Wnt-3a [Unconjugated]](https://images.novusbio.com/images/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-252023103514.jpg)
![Bioactivity GDNF [Unconjugated]](https://images.novusbio.com/images2/GDNF_212GD_2846.jpg)
![SDS-PAGE GDNF [Unconjugated]](https://images.novusbio.com/images2/GDNF_212-GD_62.jpg)
![SEC-MALS GDNF [Unconjugated]](https://images.novusbio.com/images/212-gd_recombinant-human-gdnf-protein-sec-mals-25202393740..jpg)
![Western Blot Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images2/Wnt-7b_AF3460_Western_Blot_11133.jpg)
![Immunohistochemistry Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af3460_human-wnt-7b-affinity-purified-polyclonal-ab-immunohistochemistry-12122023155533..jpg)
![Western Blot Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images/af3460_human-wnt-7b-affinity-purified-polyclonal-ab-41202413392421.jpg)
![Western Blot BMP-1/PCP Antibody [Unconjugated]](https://images.novusbio.com/images2/BMP1_AF1927_Western_Blot_21018.jpg)
![Western Blot BMP-1/PCP Antibody [Unconjugated]](https://images.novusbio.com/images2/BMP1_AF1927_Western_Blot_21019.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")