| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant mouse Wnt‑2b Leu64-Glu104 and Arg251-Ala312 Accession # O70283 |
| Specificity | Detects recombinant human and recombinant mouse Wnt-2b in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-7a, -9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, and -16 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | WNT2B |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
![Wnt-2b Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Wnt-2b Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Bioactivity Wnt-5a [Unconjugated]](https://images.novusbio.com/images/protein/645-wn_recombinant-human-mouse-wnt-5a-protein-bioactivity-1612202094911.jpg)
![Western Blot Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images2/Wnt-7b_AF3460_Western_Blot_11133.jpg)
![Immunohistochemistry Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af3460_human-wnt-7b-affinity-purified-polyclonal-ab-immunohistochemistry-12122023155533..jpg)
![Western Blot Wnt-7b Antibody [Unconjugated]](https://images.novusbio.com/images/af3460_human-wnt-7b-affinity-purified-polyclonal-ab-41202413392421.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/Wnt3a_5036WN_2880.jpg)
![Bioactivity Wnt-3a [Unconjugated]](https://images.novusbio.com/images2/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-3112023115329.jpg)
![Cell Culture Wnt-3a [Unconjugated]](https://images.novusbio.com/images/5036-wn_recombinant-human-wnt-3a-protein-bioactivity-252023103514.jpg)
