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WBP4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: WBP4 Antibody [NBP1-84718] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Western Blot: WBP4 Antibody [NBP1-84718] - Analysis in human cell line HEL.
Immunohistochemistry-Paraffin: WBP4 Antibody [NBP1-84718] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: WBP4 Antibody [NBP1-84718] -Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: WBP4 Antibody [NBP1-84718] -Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: WBP4 Antibody [NBP1-84718] -Staining of human small intestine shows strong nuclear positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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WBP4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YCKCWIADNRPSVEFHERGKNHKENVAKRISEIKQKSLDKAKEEEKASKEFAAMEAAALKAYQEDLKRLGLESEILEPSITPVTSTIPPTSTSNQQKEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WBP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
WBP4 Protein (NBP1-84718PEP)

Reactivity Notes

Mouse (87%), Rat (88%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for WBP4 Antibody

  • domain-containing binding protein 4
  • WW domain binding protein 4 (formin binding protein 21)

Background

The WBP4 gene encodes WW domain-containing binding protein 4. The WW domain represents a small and compact globular structure that interacts with proline-rich ligands. This encoded protein is a general spliceosomal protein that may play a role in cross-intron

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol WBP4