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FNBP4 Antibody

Images

 
Western Blot: FNBP4 Antibody [NBP2-14022] - Analysis in control (vector only transfected HEK293T lysate) and FNBP4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: FNBP4 Antibody [NBP2-14022] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunohistochemistry-Paraffin: FNBP4 Antibody [NBP2-14022] - Staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FNBP4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: NGETAIGAENSEKIDENSDKEMEVEESPEKIKVQTTPKVEEEQDLKFQIGELANTLTSKFEFLGINRQSISNFHVLLLQTETRIAD
Predicted Species
Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FNBP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunofluorescence, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
FNBP4 Protein (NBP2-14022PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FNBP4 Antibody

  • DKFZp686M14102
  • FBP30
  • FLJ41904
  • formin binding protein 4
  • Formin-binding protein 30
  • formin-binding protein 4
  • KIAA1014DKFZp779I1064

Background

Partial sequence of FNBP4/FBP30 was originally isolated in a screen for proteins that interact with formins, proteins involved in murine limb and kidney development. The full length FNBP4/FBP30 was identified in a screen for genes upregulated in irradiated thymocytes. FNBP4/FBP30 bears two WW domains, a domain that resembles the src-homology 3 (SH3) domain and is known to associate with proline-rich motifs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FNBP4 Antibody (NBP2-14022) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FNBP4