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Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor® 525]

Name: Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor&#174; 525]
Brand: Novus Biologicals
SKU: NBP2-74851JF525
Price: 669 USD

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Product Details

Summary

Reactivity	Hu, Mu, RtSpecies Glossary
Applications	WB, IHC
Clone	OTI1E1
Clonality	Monoclonal
Host	Mouse
Conjugate	Janelia Fluor 525

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NBP2-74851AF350

Alexa Fluor 405 NBP2-74851AF405

Alexa Fluor 488 NBP2-74851AF488

Alexa Fluor 532 NBP2-74851AF532

Alexa Fluor 594 NBP2-74851AF594

Alexa Fluor 647 NBP2-74851AF647

Alexa Fluor 700 NBP2-74851AF700

Alexa Fluor 750 NBP2-74851AF750

Biotin NBP2-74851B

CoraFluor 1 NBP2-74851CL1

DyLight 350 NBP2-74851UV

DyLight 405 NBP2-74851V

DyLight 488 NBP2-74851G

DyLight 550 NBP2-74851R

DyLight 594 NBP2-74851DL594

DyLight 650 NBP2-74851C

DyLight 680 NBP2-74851FR

DyLight 755 NBP2-74851IR

FITC NBP2-74851F

HRP NBP2-74851H

Janelia Fluor 549 NBP2-74851JF549

Janelia Fluor 646 NBP2-74851JF646

Janelia Fluor 669 NBP2-74851JF669

Unconjugated NBP2-45411

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation NBP2-45411

Azide and BSA Free NBP2-74851

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74851AF350
Alexa Fluor 405	NBP2-74851AF405
Alexa Fluor 488	NBP2-74851AF488
Alexa Fluor 532	NBP2-74851AF532
Alexa Fluor 594	NBP2-74851AF594
Alexa Fluor 647	NBP2-74851AF647
Alexa Fluor 700	NBP2-74851AF700
Alexa Fluor 750	NBP2-74851AF750
Biotin	NBP2-74851B
CoraFluor 1	NBP2-74851CL1
DyLight 350	NBP2-74851UV
DyLight 405	NBP2-74851V
DyLight 488	NBP2-74851G
DyLight 550	NBP2-74851R
DyLight 594	NBP2-74851DL594
DyLight 650	NBP2-74851C
DyLight 680	NBP2-74851FR
DyLight 755	NBP2-74851IR
FITC	NBP2-74851F
HRP	NBP2-74851H
Janelia Fluor 549	NBP2-74851JF549
Janelia Fluor 646	NBP2-74851JF646
Janelia Fluor 669	NBP2-74851JF669
Unconjugated	NBP2-45411

Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor® 525] Summary

Immunogen	Full length human recombinant protein of human VHL(NP_000542) produced in HEK293T cell.
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	VHL
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Immunohistochemistry Immunohistochemistry-Paraffin Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor® 525]

elongin binding protein
HRCA1
Protein G7
pVHL
RCA1
VHL
VHL1
von Hippel-Lindau disease tumor suppressor
von Hippel-Lindau syndrome
von Hippel-Lindau tumor suppressor

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.

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Bioinformatics

Gene Symbol

VHL

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor® 525]

Species Glossary

Images

Product Details

Order Details

Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor® 525] Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Notes

Alternate Names for Von Hippel Lindau Antibody (OTI1E1) [Janelia Fluor® 525]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Von Hippel Lindau Antibody (NBP2-74851JF525) (0)

Reviews for Von Hippel Lindau Antibody (NBP2-74851JF525) (0)

Product General Protocols

Video Protocols

FAQs for Von Hippel Lindau Antibody (NBP2-74851JF525) (0)

Secondary Antibodies

Isotype Controls

Additional Von Hippel Lindau Products

Research Areas for Von Hippel Lindau Antibody (NBP2-74851JF525)

Blogs on Von Hippel Lindau

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Bioinformatics