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Human Vitronectin ELISA Kit (Colorimetric)

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ELISA: Human Vitronectin ELISA Kit (Colorimetric) [NBP2-80358] - Example standard curve for Human Vitronectin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human Vitronectin ELISA Kit (Colorimetric) [NBP2-80358] - Example standard curve for Human Vitronectin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP
Suitable Sample Type
Serum
Standard Curve Range
31.25-2000 pg/ml (example only; lot dependent)
Sensitivity
15.19 pg/ml

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Human Vitronectin ELISA Kit (Colorimetric) Summary

Specificity
Recognizes both recombinant and natural Human Vitronectin.
Standard Curve Range
31.25-2000 pg/ml (example only; lot dependent)
Sensitivity
15.19 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 2.4 %
Intra-Assay
CV% < 6.2
Spike Recovery
96 -110%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
VTN

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Kit Components

Components
  1. 1. 96 well microplate coated with Capture Antibody
  2. 2. Detection Antibody conjugated to HRP
  3. 3. Standards
  4. 4. Wash Buffer Concentrate
  5. 5. Dilution Buffer Concentrate
  6. 6. Color Reagent A
  7. 7. Color Reagent B
  8. 8. Stop Solution

Alternate Names for Human Vitronectin ELISA Kit (Colorimetric)

  • Complement S-protein
  • epibolin
  • Serum Spreading Factor
  • Serum-spreading factor
  • Somatomedin B
  • S-protein
  • V75
  • vitronectin (serum spreading factor, somatomedin B, complement S-protein)
  • Vitronectin
  • VN
  • VNT
  • VTN

Background

Vitronectin also called serum spreading factor or complement S-protein, is a member of the pexin family (1). Found in serum and in tissues, Vitronectin exist in two forms; as a single chain (75kDa) and as a clipped form composed of two chains (65kDa and 10kDa). Vitronectin promotes cell adhesion, spreading and migration through specific interactions with cellular integrins, and inhibits cytolysis by the complement C5b-9 complex (2-4). Vitronectin also interacts with several critical proteins that regulate thrombosis and fibrinolysis. Vitronectin is phosphorylated by PKA and CKII (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Manual - Human Vitronectin ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol VTN