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Villin 1 Antibody (VIL1/1325) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
VIL1/1325
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-54515AF350
Alexa Fluor 405
NBP2-54515AF405
Alexa Fluor 488
NBP2-54515AF488
Alexa Fluor 532
NBP2-54515AF532
Alexa Fluor 594
NBP2-54515AF594
Alexa Fluor 647
NBP2-54515AF647
Alexa Fluor 700
NBP2-54515AF700
Alexa Fluor 750
NBP2-54515AF750
Biotin
NBP2-54515B
CoraFluor 1
NBP2-54515CL1
DyLight 350
NBP2-54515UV
DyLight 405
NBP2-54515V
DyLight 488
NBP2-54515G
DyLight 550
NBP2-54515R
DyLight 594
NBP2-54515DL594
DyLight 650
NBP2-54515C
DyLight 680
NBP2-54515FR
DyLight 755
NBP2-54515IR
FITC
NBP2-54515F
HRP
NBP2-54515H
Janelia Fluor 525
NBP2-54515JF525
Janelia Fluor 549
NBP2-54515JF549
Janelia Fluor 669
NBP2-54515JF669
Unconjugated
NBP2-53201-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-53201-100ug
Azide and BSA Free
NBP2-54515-100ug
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Villin 1 Antibody (VIL1/1325) [Janelia Fluor® 646] Summary

Immunogen
Recombinant human Villin 1 fragment (around aa179-311) (exact sequence is proprietary)) (Uniprot: P09327)
Localization
Cytoplasmic & Cell Surface
Marker
GI-Mucosal & Urogenital Brush Border Marker
Specificity
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VIL1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Villin 1 Antibody (VIL1/1325) [Janelia Fluor® 646]

  • D2S1471
  • VIL
  • villin 1
  • villin-1

Background

Villin 1 encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol VIL1