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VIAAT/SLC32A1/VGAT Antibody

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Western Blot: VIAAT/SLC32A1/VGAT Antibody [NB300-740] - Analysis of VIAAT/SLC32A1/VGAT in rat hippocampal lysate.
Western Blot: VIAAT/SLC32A1/VGAT Antibody [NB300-740] - Analysis of VIAAT/SLC32A1/VGAT in rat hippocampal lysate.

Product Details

Summary
Reactivity Mu, Rt, Ch, Xp, Bv, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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VIAAT/SLC32A1/VGAT Antibody Summary

Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # O35458
Specificity
Specific for endogenous levels of the ~53 kDa VIAAT/SLC32A1/VGAT protein.
Predicted Species
Primate (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC32A1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:100 - 1:1000
  • Western Blot 1:1000
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

It is anticipated that the antibody will also work with bovine, canine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for VIAAT/SLC32A1/VGAT Antibody

  • bA122O1.1
  • hVIAAT
  • SLC32A1
  • solute carrier family 32 (GABA vesicular transporter), member 1
  • Solute carrier family 32 member 1
  • Vesicular GABA transporter
  • vesicular inhibitory amino acid transporter
  • VGAT
  • VGATGABA and glycine transporter
  • VIAAT

Background

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SLC32A1
Entrez
Uniprot