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GAT3 Antibody

Images

 
Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.
Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebellum shows strong positivity in processes in cells in molecular layer.
Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebral cortex shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GAT3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GTLPEKLQKLTTPSTDLKMRGKLGVSPRMVTVNDCDAKLKSDGTIAAITEKETHF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC6A11
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GAT3 Protein (NBP1-91920PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GAT3 Antibody

  • GABA Transporter 3
  • GABT3
  • GAT-3
  • GAT3sodium- and chloride-dependent GABA transporter 3
  • GAT4
  • solute carrier family 6 (neurotransmitter transporter, GABA), member 11
  • Solute carrier family 6 member 11

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-3 has been found to be localized to astrocytes within the cerebral cortex indicating that this transporter mediates GABA uptake into glial cells (Minelli et al., 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC6A11