VIAAT/SLC32A1/VGAT Antibody - BSA Free

Synaptic transmission involves the regulated exocytosis of vesicles containing neurotransmitters. The inhibitory neurotransmitter g-aminobutyric acid (GABA) is synthesized in the cytoplasm from L-glutamate by the enzyme glutamic acid decarboxylase (GAD) and loaded in synaptic vesicles for exocytosis. The vesicular GABA transporter (VGAT, also termed vesicular inhibitory amino acid transporter or VIAAT, 52-57 kDa) is responsible for the uptake of GABA into synaptic vesicles, following synthesis by GAD, or reuptake by plasma membrane GABA transporters (GATs). VGAT protein (55 kDa) contains ten transmembrane domains and belongs to a new family of vesicular neurotransmitter transporters. VGAT is highly conserved in mouse, rat and human (95-98% identity). VGAT uses both chemical and electrical components of the luminal protein gradient to mediate the accumulation of GABA into synaptic vesicles. VGAT is also responsible for glycine uptake in glycinergic neurons. VGAT is a homolog of the c. elegans unc -47 protein involved in GABA transport. Unc-47 is expressed in GABA neurons, localizes to synaptic vesicles and confers vesicular GABA transport in transfected cells. Mutants of c. elegans in which the unc-47 gene encoding VGAT is non-functional or absent, exhibit a complete loss of GABAergic function and elevated levels of GABA in the cytoplasm. VGAT is present in nerve endings of inhibitory neurons containing GABA, but also in glycinergic neurons in the brain and retina.1,4,5 At subcellular levels, VGAT specifically associates with synaptic vesicles. VGAT/VIAAT is also expressed in rat pancreatic islet cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ELISA, WB.

Showing Publications 1 - 4 of 4.

Publications using NB110-55238	Applications	Species
Arsenault D, Tremblay C, Emond V, Calon F Sex-dependent alterations in the physiology of entorhinal cortex neurons in old heterozygous 3xTg-AD mice Biol Sex Differ 2020-11-16 [PMID: 33198813] (WB, Mouse)	WB	Mouse
Bories C, Arsenault D, Lemire M et al. Transgenic autoinhibition of p21-activated kinase exacerbates synaptic impairments and fronto-dependent behavioral deficits in an animal model of Alzheimer's disease. Aging (Albany NY). 2017-05-16 [PMID: 28522792] (WB, Mouse)	WB	Mouse
Gruol Donna L, Vo Khanh, Bray Jennifer G, Roberts Amanda J. CCL2-ethanol interactions and hippocampal synaptic protein expression in a transgenic mouse model. Front Integr Neurosci. 2014-04-04 [PMID: 24772072] (ELISA, Mouse)	ELISA	Mouse
Nelson TE, Olde Engberink A, Hernandez R et al. Altered synaptic transmission in the hippocampus of transgenic mice with enhanced central nervous systems expression of interleukin-6. Brain Behav Immun. 2012-05-17 [PMID: 22609298] (WB, Mouse)	WB	Mouse

Array NB110-55238

• VIAAT/SLC32A1/VGAT Antibodies
• VIAAT/SLC32A1/VGAT Lysate
• VIAAT/SLC32A1/VGAT Proteins