![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249393566.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1615 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human VIAAT/SLC32A1/VGAT. The exact sequence is proprietary. |
| Predicted Species | Rhesus Macaque (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | SLC32A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for VIAAT/SLC32A1/VGAT Antibody (NBP3-25742)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC32A1 |
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024941475.jpg "Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249415931.jpg "Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024943286.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249383131.jpg "Immunocytochemistry/Immunofluorescence: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")