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VASA Antibody (2F9H5) - BSA Free

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Western Blot: VASA Antibody (2F9H5) [NBP1-51586] - Analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
Flow Cytometry: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
2F9H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price
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VASA Antibody (2F9H5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human VASA expressed in E. Coli.
Marker
Primordial Germ Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDX4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for VASA Antibody (2F9H5) - BSA Free

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VASA Antibody (NBP1-51586) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VASA Antibody (NBP1-51586). (Showing 1 - 1 of 1 FAQ).

  1. Has the staining of NTERA-2 cells using DDX4 mouse mAb been shown in a publication?
    • Despite being one of our best sellers, this antibody has not yet been cited in publications to our knowledge. However, we stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol DDX4
Entrez
Uniprot