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VAP-1/AOC3 Antibody (174-5)

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Immunohistochemistry-Paraffin: VAP1 Antibody (174-5) [NBP1-58375] - Analysis of VAP1 on human tonsil tissue. Anti-human VAP1 staining results in vessels that are VAP1 positive, whereas morphologically similar vessels next to postive ones can be negative.
Immunohistochemistry-Paraffin: VAP1 Antibody (174-5) [NBP1-58375] - Analysis of VAP1 on human tonsil tissue. Anti-human VAP1 staining results in vessels that are VAP1 positive, whereas morphologically similar vessels ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
174-5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

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VAP-1/AOC3 Antibody (174-5) Summary

Immunogen
The monoclonal antibody 174-5 cross-reacts with rat VAP-1. The monoclonal antibody inhibits lymphocyte infiltration in liver allograft rejection in rats.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AOC3
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Publications
Read Publication using
NBP1-58375 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for VAP-1/AOC3 Antibody (174-5)

  • amine oxidase, copper containing 3 (vascular adhesion protein 1)
  • AOC3
  • Copper amine oxidase
  • EC 1.4.3
  • HPAO
  • HPAOSSAO
  • Semicarbazide-sensitive amine oxidase
  • SSAO
  • VAP1
  • VAP-1
  • VAP1EC 1.4.3.21
  • VAP-1membrane primary amine oxidase
  • Vascular adhesion protein 1

Background

The monoclonal antibody 174-5 recognises human Vascular Adhesion Protein-1 (VAP-1) also known as AOC3, a heavily sialylated homodimeric glycoprotein of 180 kD. VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation. The monoclonal antibody 174-5 cross-reacts with rat VAP-1. The monoclonal antibody inhibits lymphocyte infiltration in liver allograft rejection in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAP-1/AOC3 Antibody (NBP1-58375)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.


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FAQs for VAP-1/AOC3 Antibody (NBP1-58375). (Showing 1 - 1 of 1 FAQ).

  1. One of our customers is very interested in your Antibody: VAP1 Antibody (174-5). This antibody is not listed as functional assays, but in the reference cited in the data sheet, there is an article referring this antibody as blocking VAP-1 in vivo in rats. The article is: Martelius, T et al. Blockade of vascular adhesion protein-1 inhibits lymphocyte infiltration in rat liver allograft rejection. Am J Path 2004, 165: 199. Could you please confirm that this antibody could be used in Functional assays? Also the format is really small and the customer need huge quantity of antibody as the recommended dosys is 2mg/kg. Do you have any other bigger format?
    • This antibody has not been tested in functional assays. It has been validated for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry-Frozen. The reference you refer to has been taken off our website. I apologize for any confusion.

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Bioinformatics

Gene Symbol AOC3