LOX Antibody

LOX (lysyl oxidase) is a copper-dependent amine oxidase enzyme responsible for post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. LOX is synthesized as an inactive 50 kD proenzyme that is N-glycosylated in ER/Golgi complex and then secreted into the extracellular environment, where it is processed by pro-collagen C-proteinases (mammalian Tolloids and BMP-1) into an active enzyme (28-32 kD) and an 18 kD propeptide (LOX-PP) which can be N-glycosylated intracellularly into a ~35 kD form. LOX activity is critical in maintaining the tensile as well as elastic features of the connective tissues of skeletal, pulmonary and cardiovascular systems. LOX deficiency leads to intra-gestational lethality in LOX-/- mice wherein the pups exhibits aortic aneurysms/tortuosity/rupture. LOX expression is highly regulated during normal development. Aberrant expression or activity has been documented in a range of diseases predominantly associated with the ECM such as Cutis laxa or OHS (occipital horn syndrome), Menkes' syndrome, myocardial ischaemia/heart failure, atherosclerosis, scleroderma, liver cirrhosis, Pseudoexfoliation syndrome and glaucoma, Alzheimer's/non-Alzheimer's dementia, Wilson's disease, primary biliary cirrhosis as well as in many type of cancers, wherein LOX implicates in promoting tumor cell invasion and metastasis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Feline, Porcine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.

Showing Publications 1 - 10 of 36. Show All 36 Publications. Collapse Publications.

Publications using NB100-2527	Applications	Species
Cheng W, Lawson C, Liu H et al. Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy Animals 2023-06-26 [PMID: 37443910] (IHC-P, Feline)	IHC-P	Feline
Sasaki M, Hara T, Wang JX et al. Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitis bioRxiv : the preprint server for biology 2023-03-28 [PMID: 37034590] (WB)	WB
Wang Q, Sundar IK, Lucas JH et al. Circadian clock molecule REV-ERB? regulates lung fibrotic progression through collagen stabilization Nature communications 2023-03-09 [PMID: 36894533] (IHC-P, WB, Mouse, Human)	IHC-P, WB	Mouse, Human
Ning Y, Zhou IY, Roberts JD Jr et al. Molecular MRI quantification of extracellular aldehyde pairs for early detection of liver fibrogenesis and response to treatment Science Translational Medicine 2022-09-21 [PMID: 36130015] (IHC)	IHC
Jiang C, Wang M, Yao W et al. Comprehensive Analysis on Prognosis and Immune Infiltration of Lysyl Oxidase Family Members in Pancreatic Adenocarcinoma With Experimental Verification Frontiers in Molecular Biosciences 2022-04-01 [PMID: 35433829] (IHC)	IHC
Novotny T, Eckhardt A, Doubkova M et al. The possible role of hypoxia in the affected tissue of relapsed clubfoot Scientific reports 2022-03-15 [PMID: 35292718] (IF/IHC, Human)	IF/IHC	Human
Yang YL, Tsai MC, Chang YH et al. MIR29A Impedes Metastatic Behaviors in Hepatocellular Carcinoma via Targeting LOX, LOXL2, and VEGFA International Journal of Molecular Sciences 2021-06-01 [PMID: 34206143]
Cho W, Kim MS, Lee KH Ionizing radiation attracts tumor targeting and apoptosis by radiotropic lysyl oxidase traceable nanoparticles Nanomedicine 2019-12-10 [PMID: 31830613] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Xu XH, Jia Y, Zhou X et al. Downregulation of lysyl oxidase and lysyl oxidase-like protein 2 suppressed the migration and invasion of trophoblasts by activating the TGF-b/collagen pathway in preeclampsia Exp. Mol. Med. 2019-02-21 [PMID: 30804321] (IF/IHC, Human)	IF/IHC	Human
Vadasz Z, Balbir Gurman A, Meroni P et al. Lysyl oxidase-a possible role in systemic sclerosis-associated pulmonary hypertension: a multicentre study Rheumatology (Oxford) 2019-02-15 [PMID: 30770717] (ICC/IF, Human)	ICC/IF	Human
Wei MM, Wang SS, Zheng JL et al. Herbal compound Teng-Long-Bu-Zhong-Tang inhibits metastasis in human RKO colon carcinoma Oncol Lett. 2017-10-17 [PMID: 29344222] (IHC-P, Human)	IHC-P	Human
De Donato M, Petrillo M, Martinelli E et al. Uncovering the role of nuclear Lysyl oxidase (LOX) in advanced high grade serous ovarian cancer. Gynecol. Oncol. 2017-05-08 [PMID: 28495238] (IF/IHC, Human)	IF/IHC	Human
Gao AE, Sullivan KE, Black LD. Lysyl oxidase expression in cardiac fibroblasts is regulated by alpha2beta1 integrin interactions with the cellular microenvironment. Biochem. Biophys. Res. Commun. 2016-06-17 [PMID: 27169768] (IF/IHC, WB, Rat)	IF/IHC, WB	Rat
Nilsson G, Kannius-Janson M. Forkhead Box F1 promotes breast cancer cell migration by upregulating lysyl oxidase and suppressing Smad2/3 signaling. BMC Cancer 2016-02-23 [PMID: 26908052] (WB)	WB
Nilsson M, Adamo H, Bergh A, Halin Bergstrom S Inhibition of Lysyl Oxidase and Lysyl Oxidase-Like Enzymes Has Tumour-Promoting and Tumour-Suppressing Roles in Experimental Prostate Cancer. Sci Rep 2016-01-25 [PMID: 26804196] (IF/IHC)	IF/IHC
Herum K M, Lunde I G et al. Syndecan-4 is a key determinant of collagen cross-linking and passive myocardial stiffness in the pressure-overloaded heart. Cardiovasc Res 2015-01-05 [PMID: 25587045] (WB, Mouse)	WB	Mouse
Camasao DB, Li L, Drouin B et al. Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation In vitro models 2022-01-01
Ma W, Li T, Wu S et al. LOX and ACSL5 as potential relapse markers for pancreatic cancer patients Cancer Biol. Ther. 2019-02-03 [PMID: 30712446] (IF/IHC, Human)	IF/IHC	Human
Kasagi Y, Dods K, Wang JX et al. Fibrostenotic eosinophilic esophagitis may reflect epithelial lysyl oxidase induction by fibroblasts-derived tumor necrosis factor-a J. Allergy Clin. Immunol. 2018-12-19 [PMID: 30578874] (IHC-P, Human)	IHC-P	Human
Joung Jae Won, Oh Hoon Kyu, Lee Sun Jae et al. Significance of Intratumoral Fibrosis in Clear Cell Renal Cell Carcinoma. Journal of Pathology and Translational Medicine 2018-08-19 [PMID: 30121971] (IF/IHC, Human)	IF/IHC	Human
Pezzoli D, Di Paolo J, kumra H et al. Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds. Biomaterials. 2018-07-12 [PMID: 30036726] (IHC-P, Porcine)	IHC-P	Porcine
Dodson RB, Miller TA, Powers KN et al. Intrauterine Growth Restriction Influences Vascular Remodeling and Stiffening in the Weanling Rat More than Sex or Diet. Am. J. Physiol. Heart Circ. Physiol. 2016-11-23 [PMID: 27881387] (Rat)		Rat
Heeg S, Das K K et al. ETS-Transcription Factor ETV1 Regulates Stromal Expansion and Metastasis in Pancreatic Cancer. Gastroenterology 2016-01-09 [PMID: 27318148] (IF/IHC, Mouse)	IF/IHC	Mouse
Hinderer S, Shena N, Ringuette LJ et al. In vitro elastogenesis: instructing human vascular smooth muscle cells to generate an elastic fiber-containing extracellular matrix scaffold. Biomed Mater 2015-06-01 [PMID: 25784676] (Human)		Human
Ovet H, Oztay F. The Copper Chelator Tetrathiomolybdate Regressed Bleomycin-Induced Pulmonary Fibrosis in Mice, by Reducing Lysyl Oxidase Expressions. Biol Trace Elem Res. 2014-10-28 [PMID: 25349139] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Shen CJ, Sharma A, Vuong DV et al. Ionizing radiation induces tumor cell lysyl oxidase secretion. BMC Cancer 2014-07-22 [PMID: 25052686] (IHC-P, WB, Human) Details: LOX antibody used for WB in conditioned medium from multiple human tumor cell lines in response to irradiation/hypoxia and treatment with microtubule stabilizing agent Patupilone (Epothilone B, EPO906) and in IHC-P on A549-derived tumor xenografts treated with irradiation. WB images shows the detection of LOX zymogen (50 kD) and active form (32 kD) - Figures 1, 3, 4.	IHC-P, WB	Human
Shen J, Xia W, Khotskaya YB et al. EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Nature. 2013-05-16 [PMID: 23636329] (WB, Human)	WB	Human
Dumont N, Liu B, Defilippis RA et al. Breast fibroblasts modulate early dissemination, tumorigenesis, and metastasis through alteration of extracellular matrix characteristics. Neoplasia 2013-03-01 [PMID: 23479504] (Human)		Human
Chou J, Lin JH, Brenot A et al. GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression. Nat Cell Biol 2012-12-23 [PMID: 23354167] (WB, Mouse)	WB	Mouse
Teppo S, Sundquist E, Vered M et al. The hypoxic tumor microenvironment regulates invasion of aggressive oral carcinoma cells Exp Cell Res 2012-12-19 [PMID: 23262025] (WB, Human)	WB	Human
Herum KM, Lunde IG, Skrbic B et al. Syndecan-4 signaling via NFAT regulates extracellular matrix production and cardiac myofibroblast differentiation in response to mechanical stress Journal of Molecular and Cellular Cardiology 2013-01-01 [PMID: 23178899] (IHC, WB)	IHC, WB
Mizuno S, Yasuo M, Bogaard HJ et al. Inhibition of histone deacetylase causes emphysema Am J Physiol Lung Cell Mol Physiol 2011-03-01 [PMID: 21224215] (WB, Rat)	WB	Rat
Zibadi S, Vazquez R, Moore D et al. Myocardial lysyl oxidase regulation of cardiac remodeling in a murine model of diet-induced metabolic syndrome. Am J Physiol Heart Circ Physiol;297(3):H976-982. 2009-01-01 [PMID: 19592613]
Contente S, Yeh TJ, Friedman RM. Tumor suppressive effect of lysyl oxidase proenzyme. Biochim Biophys Acta 2009-07-01 [PMID: 19410608]
Ovchinnikova O, Robertson A-KL, Wagsater D et al. T-Cell Activation Leads to Reduced Collagen Maturation in Atherosclerotic Plaques of Apoe-/- Mice. Am J Pathol;174(2):693-700. 2009-01-01 [PMID: 19131590]
Schlotzer-Schrehardt U et al. Genotype-Correlated Expression of Lysyl Oxidase-Like 1 in Ocular Tissues of Patients with Pseudoexfoliation Syndrome/Glaucoma Normal Patients. Am J Pathol;173(6):1724-1735. 2008-01-01 [PMID: 18974306]
Show All 36 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Wan-Ching Cheng	IHC-P	cat and Rat	08/03/2018	View Summary Application Immunohistochemistry-Paraffin Sample Tested IHC-P Sample Tested Species cat and Rat Lot H-5
Enlarge	4 reviewed by: Verified Customer	IF	Human	06/07/2018	View Summary Application Immunofluorescence Sample Tested fibroblasts Species Human Lot H-3
Enlarge	4 reviewed by: Verified Customer	IHC-P	Mouse	01/14/2015	View Summary Application Immunohistochemistry-Paraffin Sample Tested mouse lung Species Mouse Lot G-1
Enlarge	4 reviewed by: Saray Varona	WB	Mouse	03/14/2014	View Summary Application Western Blot Sample Tested mouse vascular smooth muscle cell Species Mouse Lot G-1
	4 reviewed by: Verified Customer	WB	Human	06/19/2009	View Summary Application Western Blot Sample Tested Renal cell carcinoma cell line UMRC2, Sample Amount: 100ug Species Human Lot A

Array NB100-2527

• LOX Antibodies
• LOX Lysate
• LOX Proteins