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VAC14 Antibody

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Western Blot: VAC14 Antibody [NBP2-44278] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44278 was used for IP at 6 ug per reaction. VAC14 was also Immunoprecipitated by another rabbit anti-VAC14 antibody.
Western Blot: VAC14 Antibody [NBP2-44278] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44278 ...read more
Independent Antibodies: Western Blot: VAC14 Antibody [NBP2-44278] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer.

Product Details

Summary
Reactivity Hu, Rt, BvSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-44278AF350
Alexa Fluor 405
NBP2-44278AF405
Alexa Fluor 488
NBP2-44278AF488
Alexa Fluor 532
NBP2-44278AF532
Alexa Fluor 594
NBP2-44278AF594
Alexa Fluor 647
NBP2-44278AF647
Alexa Fluor 700
NBP2-44278AF700
Alexa Fluor 750
NBP2-44278AF750
Biotin
NBP2-44278B
CoraFluor 1
NBP2-44278CL1
DyLight 350
NBP2-44278UV
DyLight 405
NBP2-44278V
DyLight 488
NBP2-44278G
DyLight 550
NBP2-44278R
DyLight 594
NBP2-44278DL594
DyLight 650
NBP2-44278C
DyLight 680
NBP2-44278FR
DyLight 755
NBP2-44278IR
HRP
NBP2-44278H
Janelia Fluor 525
NBP2-44278JF525
Janelia Fluor 549
NBP2-44278JF549
Janelia Fluor 585
NBP2-44278JF585
Janelia Fluor 635
NBP2-44278JF635
Janelia Fluor 646
NBP2-44278JF646
Janelia Fluor 669
NBP2-44278JF669
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VAC14 Antibody Summary

Immunogen
The epitope recognized by this antibody maps to a region between residue 732 to 782 of human Protein VAC14 homolog using the numbering given in entry Q08AM6.1 (GeneID 55697).
Predicted Species
Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VAC14
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAC14 Antibody

  • ArPIKfyve
  • FLJ10305
  • FLJ36622
  • MGC149816
  • protein VAC14 homolog
  • Tax1 (human T-cell leukemia virus type I) binding protein 1
  • Tax1 (human T-cell leukemia virus type I) binding protein 2
  • Tax1-binding protein 2
  • TAX1BP2FLJ46582
  • TRXMGC149815
  • Vac14 homolog (S. cerevisiae)

Background

Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a low-abundance signaling molecule. A regulatory complex made up of VAC14 and FIG4 (MIM 609390) control synthesis of PI(3,5)P2 by activating PI(3)P kinase, FAB1 (PIP5K3; MIM 609414). The VAC14/FIG4 complex also functions in the breakdown of PI(3,5)P2 (Zhang et al., 2007 [PubMed 17956977]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-44278
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Applications: WB, IP

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol VAC14