Novus Biologicals products are now on bio-techne.com

UBR4 Antibody

Images

 
Western Blot: UBR4 Antibody [NBP1-28730] - Whole cell lysate from NIH3T3, TCMK-1, 4T1, and CT26.WT cells.
Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p600 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: UBR4 Antibody [NBP1-28730] - Detection of Human p600 on HeLa whole cell lysate using NBP28730. p600 was also immunoprecipitated by rabbit anti-p600 antibodies ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

UBR4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1350 and 1400 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBR4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:500 to 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NBP1-28730 in the following applications:

  • IB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBR4 Antibody

  • 600 kDa retinoblastoma protein-associated factor
  • EC 6.3.2
  • EC 6.3.2.-
  • KIAA0462E3 ubiquitin-protein ligase UBR4
  • KIAA1307zinc finger, UBR1 type 1
  • N-recognin-4
  • p600
  • RBAF600FLJ41863
  • Retinoblastoma-associated factor of 600 kDa
  • ubiquitin protein ligase E3 component n-recognin 4
  • Zinc finger UBR1-type protein 1
  • ZUBR1retinoblastoma-associated factor 600-like protein

Background

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-13943
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-01264
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-84255
Species: Hu
Applications: IHC, IHC-P
NBP1-91642
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-616
Species: Hu, Mu, Md, Pm, Rt
Applications: ChIP, EM, Flow-IC, Flow, ICC/IF, IP, In vitro, KD, WB
NBP1-32870
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
6507-IL/CF
Species: Hu
Applications: BA
NBP2-15104
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF5866
Species: Hu
Applications: IHC, WB
NBP2-00900
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-32312
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-85889
Species: Hu
Applications: WB
NBP1-85511
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00001523-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-88829
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
DY413
Species: Mu
Applications: ELISA
NBP1-28730
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IP

Publications for UBR4 Antibody (NBP1-28730)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IB.


Filter By Application
IB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for UBR4 Antibody (NBP1-28730) (0)

There are no reviews for UBR4 Antibody (NBP1-28730). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UBR4 Antibody (NBP1-28730) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our UBR4 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol UBR4
Entrez
Uniprot