![Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Western-Blot-NBP2-12882-img0003.jpg "Western Blot: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - analysis of Human Cell lysates showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone 6C11-B3 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 6C11-B3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Native bovine ubiquitin, conjugated to KLH |
| Localization | Cytoplasm , Nucleus |
| Specificity | Detects approx 10kDa. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UBB |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of Ubiquitin Antibody was sufficient for detection of ubiquitin in 10 ug of Hela Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Ubiquitin B Antibody (NBP2-12882)Find related products by research area.
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| Gene Symbol | UBB |
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0005.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.")
![Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.](http://images.novusbio.com/fullsize/Ubiquitin-B-Antibody-6C11-B3-Immunocytochemistry-Immunofluorescence-NBP2-12882-img0007.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin B Antibody (6C11-B3) [NBP2-12882] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin B Monoclonal Antibody, Clone 6C11-B3 (NBP2-12882). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Ubiquitin B Monoclonal Antibody (NBP2-12882) at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin B Antibody. (C) Composite.")
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