| Reactivity | HuSpecies Glossary |
| Applications | WB, CyTOF-ready, Flow |
| Clone | 488915 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human TSC1 Asp156-Thr300 Accession # Q92574 |
| Specificity | Detects human TSC1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TSC2 (aa 550-850), rhTSC2 (aa 1506-1748), or recombinant mouse TSC22 (aa 1-143) is observed. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TSC1 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
TSC1 (Tuberous sclerosis 1), or hamartin, is a tumor suppressor which interacts with tumor suppressor TSC2 (tuberin) to form a cytoplasmic heterodimer. Mutations in either hamartin or tuberin are responsible for tuberous sclerosis (TSC), an autosomal dominant disease characterized by renal dysfunction, seizures, developmental delays, benign hamartomas and low grade neoplasms predominantly affecting the CNS, kidney, lung, skin, and heart. The TSC1/TSC2 complex suppresses cell growth by inhibiting mTOR, with TSC1 acting to inhibit the ubiquitination of TSC2, leading to increased cellular levels of TSC2 and thus enhancing its catalytic activity as a GTPase-activating protein for Rheb. TSC1 and TSC2 are also involved in the G2/M transition of the cell cycle through their interactions with CDK1 and cyclin B1. TSC1 has also been shown to interact with F-actin and ERM (Ezrin-Radixin-Moesin) proteins, implying a role in the modulation of cell adhesion and morphology.
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| Publication using MAB4379 | Applications | Species |
|---|---|---|
| Zhao J, Zhao D, Poage G, Mazumdar A, Zhang Y, Hill J, Hartman Z, Savage M, Mills G, Brown P Death-associated protein kinase 1 promotes growth of p53-mutant cancers. J Clin Invest, 2015-06-15;125(7):2707-20. 2015-06-15 [PMID: 26075823] (Immunoprecipitation, Human) | Immunoprecipitation | Human |
Secondary Antibodies |
Isotype Controls |
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TSC1 - a negative regulator of mTOR signaling TSC1 is a tumor suppressor gene that encodes a 130 kDa protein called hamartin. TSC1 was first identified as an oncogenic driver of Tuberous Sclerosis, a condition characterized by numerous benign tumors of the skin, brain, heart, and lungs. A mut... Read full blog post. |
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