Novus Biologicals products are now on bio-techne.com

Troponin T Type 2 (cardiac) Antibody (CL13403)

Images

 
Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Staining of human small intestine shows no positivity in glandular cells as expected.
Orthogonal Strategies: Analysis in human heart muscle and skeletal muscle tissues using NBP3-24557 antibody. Corresponding TNNT2 RNA-seq data are presented for the same tissues.
Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.
Staining in RH-30 cell line with Anti-TNNT2 monoclonal antibody, showing specific staining of cytosol in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Western blot analysis in human heart tissue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL13403
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Troponin T Type 2 (cardiac) Antibody (CL13403) Summary

Immunogen
This antibody was designed to target the recombinant protein Troponin T Type 2 (cardiac), with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNNT2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (CL13403)

  • cardiac
  • CMD1D
  • CMPD2
  • dilated 1D (autosomal dominant)
  • hypertrophic 2
  • MGC3889
  • RCM3
  • TnTc
  • troponin T type 2 (cardiac)
  • troponin T, cardiac muscle

Background

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB9096
Species: Hu
Applications: IHC, WB
AF7439
Species: Hu
Applications: IHC, WB
NBP2-35336
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-33716
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-97725
Species: Bv, Gt, Hu, Mu, Po, Rb, Rt, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
MAB6967
Species: Hu
Applications: WB
NBP1-85541
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, WB
H00004154-M02
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-88068
Species: Hu
Applications: IHC, IHC-P
NBP3-13841
Species: Hu
Applications: Flow, ICC/IF, PA
NBP2-37958
Species: Hu
Applications: IHC, IHC-P
NBP1-88071
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
H00005555-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP1-06983
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
NB200-316
Species: Bv, Hu, Mu, Po, Pm, Rb, Rt
Applications: Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
NBP2-25151
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, WB
NB300-284
Species: Ca, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00011155-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, KD, WB
NB110-85519
Species: Mu
Applications: ELISA, IHC, IP, KD, KO, WB
NBP2-19807
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Troponin T Type 2 (cardiac) Antibody (NBP3-24557) (0)

There are no publications for Troponin T Type 2 (cardiac) Antibody (NBP3-24557).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Troponin T Type 2 (cardiac) Antibody (NBP3-24557) (0)

There are no reviews for Troponin T Type 2 (cardiac) Antibody (NBP3-24557). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Troponin T Type 2 (cardiac) Antibody (NBP3-24557) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Troponin T Type 2 (cardiac) Products

Research Areas for Troponin T Type 2 (cardiac) Antibody (NBP3-24557)

Find related products by research area.

Blogs on Troponin T Type 2 (cardiac)

There are no specific blogs for Troponin T Type 2 (cardiac), but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Troponin T Type 2 (cardiac) Antibody (CL13403) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNNT2