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Troponin T Type 2 (cardiac) Antibody (CL13403)

Images

 
Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Staining of human small intestine shows no positivity in glandular cells as expected.
Orthogonal Strategies: Analysis in human heart muscle and skeletal muscle tissues using NBP3-24557 antibody. Corresponding TNNT2 RNA-seq data are presented for the same tissues.
Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.
Staining in RH-30 cell line with Anti-TNNT2 monoclonal antibody, showing specific staining of cytosol in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
Western blot analysis in human heart tissue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL13403
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Troponin T Type 2 (cardiac) Antibody (CL13403) Summary

Immunogen
This antibody was designed to target the recombinant protein Troponin T Type 2 (cardiac), with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNNT2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Troponin T Type 2 (cardiac) Antibody (CL13403)

  • cardiac
  • CMD1D
  • CMPD2
  • dilated 1D (autosomal dominant)
  • hypertrophic 2
  • MGC3889
  • RCM3
  • TnTc
  • troponin T type 2 (cardiac)
  • troponin T, cardiac muscle

Background

Does not cross-react with skeletal muscle troponin T, cTnI or TnC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNNT2