![Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).](http://images.novusbio.com/fullsize/Troponin-T-Type-2-cardiac-Antibody-1C11-Sandwich-ELISA-NB200-412-img0002.jpg "Sandwich ELISA: Troponin T Type 2 (cardiac) Antibody (1C11) [NB200-412] - Calibrating curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen: human, canine, rat, mouse Clone 1C11 for capture (1 ug/well) and Clone b 7E7 for detection (200 ng/well). Assay time: 30 min. Room temperature. Hybridoma cell line producing Clone 7E7 was raised after hybridization of myeloma cell line Sp2/0 with splenocytes of mice immunized with human tertiary Tn complex. Thus Clone 7E7 demonstrates slight cross-reaction with cTnI (about 5% based on ELISA data).")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 1C11cc |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with free human cTnT. |
| Epitope | a.a.r. 171-190 |
| Localization | Located on the thin filament of striated muscles. |
| Specificity | No cross-reaction with skeletal TnT |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TNNT2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Control |
|
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein A purified |
![Immunohistochemistry MYH7 Antibody (940906) [Unconjugated]](https://images.novusbio.com/images2/MYH7_MAB9096_Immunohistochemistry_19508.jpg)
![Western Blot MYH7 Antibody (940906) [Unconjugated]](https://images.novusbio.com/images2/MYH7_MAB9096_Western_Blot_19525.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Troponin T Type 2 (cardiac) Antibody (NB200-412)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")