![Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against HEK293 (1) and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Antibody-2F12G2-Western-Blot-NBP2-37536-img0003.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against HEK293 (1) and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | 2F12G2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Troponin I type 2 (fast skeletal) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TNNI2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Troponin I type 2 (fast skeletal) Antibody (NBP2-37536)Find related products by research area.
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Troponin I Type 2 - I stay with fast-twitch skeletal muscles only The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac... Read full blog post. |
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| Gene Symbol | TNNI2 |
![Immunocytochemistry/Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Immunofluorescence analysis of Hela cells using TNNI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Antibody-2F12G2-Immunocytochemistry-Immunofluorescence-NBP2-37536-img0004.jpg "Immunocytochemistry/Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Immunofluorescence analysis of Hela cells using TNNI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Flow Cytometry: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Flow cytometric analysis of NIH/3T3 cells using TNNI2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Antibody-2F12G2-Flow-Cytometry-NBP2-37536-img0005.jpg "Flow Cytometry: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Flow cytometric analysis of NIH/3T3 cells using TNNI2 mouse mAb (green) and negative control (red).")
![Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 21 kDa)](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Antibody-2F12G2-Western-Blot-NBP2-37536-img0002.jpg "Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 21 kDa)")
![ELISA: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Troponin-I-type-2-fast-skeletal-Antibody-2F12G2-ELISA-NBP2-37536-img0001.jpg "ELISA: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")