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Troponin I Type 1 (slow skeletal) Antibody

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Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Western-blots for a selection of differentially-expressed proteins. MyH2b, Troponin I Type 1 (slow skeletal) (TNNI1) and MCK were used as target ...read more
Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human Skeletal Muscle, 5.0ug/ml.
Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml
Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) ...read more
Western-blots for a selection of differentially-expressed proteins.MyH2b, TNNI1 and MCK were used as target protein; GADPH was used as a control. A: Total protein from control and arginine-supplemented pig samples was ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Troponin I Type 1 (slow skeletal) Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding toTroponin I Type 1 (slow skeletal). The peptide sequence was selected from the N terminal of TNNI1 (NP_003272). Peptide sequence MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIP. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNNI1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 4-8 ug/ml
  • Western Blot 1.0 ug/ml
Application Notes
Use in ICC/IF was reported in the scientific literature (PMID: 23751911).
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-56641 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMIDs: 23751911 and 23063798). Porcine reactivity reported in scientific literature (PMID: 25635834).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Troponin I Type 1 (slow skeletal) Antibody

  • DKFZp451O223
  • slow-twitch isoform
  • SSTNI
  • TNN1
  • troponin I type 1 (skeletal, slow)
  • troponin I, skeletal, slow
  • troponin I, slow skeletal muscle

Background

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Troponin I Type 1 (slow skeletal) Antibody (NBP1-56641)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 5 applications: ICC/IF, IHC-P, Immunohistochemistry, In vivo assay, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
Immunohistochemistry
(1)
In vivo assay
(1)
WB
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Porcine
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-56641 Applications Species
Yap L, Chong LY, Tan C et al. Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs npj Regenerative Medicine 2023-05-26 [PMID: 37236990] (Immunohistochemistry, In vivo assay) Immunohistochemistry, In vivo assay
Teigen LE, Sundberg CW, Kelly LJ et al. Ca2+ dependency of limb muscle fiber contractile mechanics in young and older adults Am. J. Physiol., Cell Physiol. 2020-06-01 [PMID: 32348175] (Human) Human
Sun, Q, Peng, Y Et al. SEMA6D regulates perinatal cardiomyocyte proliferation and maturation in mice. Dev Biol 2019-08-01 [PMID: 31042497] (WB, Mouse) WB Mouse
Zhu W, Zhao M, Mattapally S et al. CCND2 Overexpression Enhances the Regenerative Potency of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Remuscularization of Injured Ventricle. Circ. Res. 2017-10-10 [PMID: 29018036] (Human) Human
Go-sha-jinki-Gan (GJG), a traditional Japanese herbal medicine, protects against sarcopenia in senescence-accelerated mice Kishida Y, Kagawa S, Arimitsu J Phytomedicine [PMID: 25636865] (WB, Mouse) WB Mouse
Ma X, Zheng C, Hu Y et al. Dietary L-arginine supplementation affects the skeletal longissimus muscle proteome in finishing pigs PLoS ONE. 2015-01-31 [PMID: 25635834] (WB, Porcine)

Details:
Troponin I Type 1 (slow skeletal) antibody used in WB application for the detection of TNNI1 protein on lysates of frozen muscle samples which were collected from control and arginine-supplemented pigs (Figure 2).
WB Porcine
Chakraborty S, Sengupta A, Yutzey KE. Tbx20 promotes cardiomyocyte proliferation and persistence of fetal characteristics in adult mouse hearts. J Mol Cell Cardiol 2013-06-07 [PMID: 23751911] (IHC-P, ICC/IF, WB, Mouse) IHC-P, ICC/IF, WB Mouse
Harmelink C, Peng Y, DeBenedittis P et al. Myocardial Mycn is essential for mouse ventricular wall morphogenesis. Dev Biol 2013-01-01 [PMID: 23063798] (WB, Mouse) WB Mouse
Ralli,S. Am. Heart J. 150 (6), 1220-1227. 2005-01-01 [PMID: 16338262]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Research Areas for Troponin I Type 1 (slow skeletal) Antibody (NBP1-56641)

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Blogs on Troponin I Type 1 (slow skeletal).

Troponin I Type 1 - Find me in your slow-twitch skeletal muscles
Troponin I is part of a heteromeric thin filament regulatory complex that is responsible for modulating skeletal and cardiac muscle contraction. Troponin complex consists of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI1
Entrez
Uniprot