![Western Blot: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199] - Staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Troponin-T-Type-3-fast-skeletal-Antibody-Western-Blot-NBP2-26199-img0002.jpg "Western Blot: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199] - Staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-TTLRSRIDQAQKHSK corresponding to C-Terminus according to NP_001036246.1, NP_001036247.1. |
| Specificity | This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | TNNT3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 35 kDa band observed in human, mouse and rat skeletal muscle lysates is reported in scientific literature (PMID: 9201030.). IHC-P: Human skeletal muscle shows staining of select fibrils. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-26199 | Applications | Species |
|---|---|---|
| Zhao N, Jiang M, Han W et al. A novel mutation in TNNT3 associated with Sheldon-Hall syndrome in a Chinese family with vertical talus. Eur J Med Genet 2011-05-01 [PMID: 21402185] |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199] - Staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Troponin-T-Type-3-fast-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP2-26199-img0001.jpg "Immunohistochemistry-Paraffin: Troponin T Type 3 (fast skeletal) Antibody [NBP2-26199] - Staining of Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
