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TRIM Antibody (TRIM-04) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
TRIM-04
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

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TRIM Antibody (TRIM-04) [Janelia Fluor® 549] Summary

Immunogen
Recombinant fragment, corresponding to amino acids 29-186 of Human TRIM.
Specificity
The antibody TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TRAT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TRIM Antibody (TRIM-04) [Janelia Fluor® 549]

  • pp29/30
  • T cell receptor associated transmembrane adaptor 1
  • T cell receptor interacting molecule
  • T-cell receptor interacting molecule
  • T-cell receptor-associated transmembrane adapter 1
  • T-cell receptor-interacting molecule
  • TCRIM
  • TRAT1
  • TRIM
  • TRIMHSPC062

Background

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit alpha after phosphorylation. TRIM has also been shown to associate with TCR alpha, beta, and zeta. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRAT1