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Human Transportin 1 ELISA Kit (Colorimetric)

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ELISA: Human Transportin 1 ELISA Kit (Colorimetric) [NBP2-76604] - Samples were spiked with high concentrations of Human Transportin 1 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Human Transportin 1 ELISA Kit (Colorimetric) [NBP2-76604] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
3.125 - 200 ng/mL
Sensitivity
1.875 ng/mL

Order Details

Human Transportin 1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human TNPO1 in samples. No significant cross-reactivity or interference between Human TNPO1 and analogues was observed.
Standard Curve Range
3.125 - 200 ng/mL
Sensitivity
1.875 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.5
Intra-Assay
%CV < 5.53
Spike Recovery
88 - 112%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNPO1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Transportin 1 ELISA Kit (Colorimetric)

  • importin beta 2
  • Importin beta-2
  • IPO2
  • karyopherin (importin) beta 2
  • MIP1Karyopherin beta-2
  • MIPM9 region interaction protein
  • transportin 1
  • transportin-1
  • TRNKPNB2importin 2

Background

Heterogeneous nuclear ribonucleoprotein hnRNPA1 is an abundant nuclear protein that plays an important role in pre-mRNA processing and mRNA export from the nucleus. A1 shuttles rapidly between the nucleus and the cytoplasm, and a 38-amino acid domain, M9, serves as the bi-directional transport signal of A1. Recently, a 90-kD protein, transportin, was identified as the mediator of A1 nuclear import. Transportin mediates the nuclear import of additional hnRNP proteins, including hnRNPF. The nuclear localization of A1 is dependent on ongoing RNA polymerase II transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Additional Transportin 1 Products

Research Areas for Transportin 1 ELISA Kit (NBP2-76604)

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Blogs on Transportin 1.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol TNPO1